Anti-UCP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P25874

Core Product Specifications and Parameters

Biological Background: UCP1 Function and Localization

• UCP1, also known as thermogenin or solute carrier family 25 member 7 (SLC25A7), is a member of the mitochondrial solute carrier family that plays a central role in non-shivering adaptive thermogenesis in brown adipose tissue and beige fat.
• It acts as a long-chain fatty acid (LCFA) and proton symporter, transporting both substrates across the inner mitochondrial membrane, which results in proton conductance that uncouples oxidative phosphorylation and converts metabolic energy into heat (PubMed:24196960, PubMed:28781081). Related references: PMID:24196960, PMID:28781081
• By dissipating the proton gradient, UCP1 regulates mitochondrial reactive oxygen species (ROS) production, contributing to cellular oxidative stress management.
• UCP1 is predominantly localized to the inner mitochondrial membrane in brown adipose tissue, where its thermogenic activity is essential for core body temperature maintenance and energy expenditure.
• This protein is involved in metabolic disorders, including obesity and diabetes mellitus, making it a potential target for therapeutic interventions aimed at modulating energy balance.
• Keywords: UCP1 is characterized by multiple transmembrane helices, ion channel activity, and roles in ion transport and oxidation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 100–200) of human UCP1 (NP_068605.1), which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), the expected band size is approximately 33 kDa; consider using lysates from brown adipose tissue or overexpressing cell lines for validation, and include proper positive and negative controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate staining in known UCP1-expressing tissues, such as brown fat depots.
• For ELISA applications, confirm antibody specificity with peptide competition assays and relevant biological matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including monoclonal/polyclonal antibodies, antibody pairs, and ancillary reagents. This anti-UCP1 antibody supports metabolic and obesity research, and our sourcing services cover rare targets and bulk custom productions. Streamline your assay development from concept to clinic with CamelBio's integrated supply chain.