Anti-UCP1 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P12242

Core Product Specifications and Parameters

Biological Background: UCP1 Function and Localization

• Mitochondrial brown fat uncoupling protein 1 (UCP1), also known as Solute carrier family 25 member 7 (Slc25a7) or Thermogenin, is encoded by the Ucp1 gene in mice.
• Functions as a long-chain fatty acid (LCFA)/proton symporter, simultaneously transporting one LCFA and one proton across the inner mitochondrial membrane, dissipating the proton gradient and converting oxidative energy into heat instead of ATP. Related references: PMID:23063128
• Plays a central role in non-shivering adaptive thermogenesis within brown adipose tissue and beige fat, responding to cold exposure and diet to regulate whole-body energy expenditure. Related references: PMID:19187776 PMID:23063128 PMID:27027295
• Modulates mitochondrial reactive oxygen species (ROS) production, thereby influencing cellular redox homeostasis. Related references: PMID:20416274 PMID:20466728
• Localizes to the mitochondrial inner membrane and contains six transmembrane helices, characteristic of the mitochondrial solute carrier family.
• Expression is predominantly restricted to brown adipose tissue, consistent with its specialized role in thermogenesis.
• Classified as a selenoprotein; incorporates selenocysteine and belongs to the mitochondrial transporter family with ion channel/transport activities.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide derived from mouse UCP1, ensuring target specificity; verify the peptide sequence if cross-reactivity is critical for your model.
• For Western blot (WB), a band at ~33 kDa is expected in mouse and rat tissue lysates; use appropriate positive controls such as brown adipose tissue.
• For immunohistochemistry (IHC-P), antigen retrieval optimization may be necessary due to the protein's membrane-embedded conformation; consider heat-mediated or enzymatic methods.
• In immunoprecipitation (IP) and ELISA, validate the antibody under your specific buffer and detection conditions to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with comprehensive IVD raw material solutions, from validated antibody pairs to bulk ancillary reagents. Explore our portfolio of mitochondrial and metabolism-related antibodies, including UCP1, for developing assays in obesity, diabetes, and thermogenesis research. Contact us for customized sourcing of rare-target monoclonals and technical support.