Anti-UCP1 Polyclonal Antibody for WB, ELISA - P10861

Core Product Specifications and Parameters

Biological Background: UCP1 Function and Localization

• UCP1, also known as Thermogenin or Solute carrier family 25 member 7, is a mitochondrial inner membrane protein primarily expressed in brown adipose tissue.
• It mediates thermogenic respiration by functioning as a long-chain fatty acid/proton symporter, dissipating the proton gradient to generate heat instead of ATP.
• UCP1 plays a critical role in non-shivering adaptive thermogenesis, regulating energy balance in response to temperature and diet variations.
• By uncoupling oxidative phosphorylation, UCP1 regulates the production of reactive oxygen species (ROS) by mitochondria.
• The protein contains multiple transmembrane helices and repeats, characteristic of the mitochondrial carrier family.
• Its molecular weight is approximately 33.4 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved intracellular region (amino acids 100-200) of bovine UCP1, suggesting potential cross-reactivity across mammals; validation in the target species is recommended.
• For Western blotting, a band at ~33 kDa is expected; consider using mitochondrial-enriched lysates from brown adipose tissue or cell lines expressing UCP1.
• In ELISA assays, this polyclonal antibody may detect denatured or native UCP1; optimization of coating buffers and antibody dilutions is advised.
• Given the antibody’s reactivity with mouse UCP1, it is suitable for studies in mouse models of thermogenesis, but confirm specificity with appropriate controls (e.g., UCP1-knockout tissues).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This UCP1 antibody supports metabolic and obesity research, and CamelBio can facilitate sourcing of rare targets and bulk reagent supply to streamline your assay development.