Anti-UCKL1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NWZ5

Core Product Specifications and Parameters

Biological Background: UCKL1 Function and Localization

• Uridine-cytidine kinase-like 1 (UCKL1), also known as UCK1L or URKL1, is a member of the uridine kinase family and exists in multiple isoforms due to alternative splicing.
• UCKL1 catalyzes the ATP-dependent phosphorylation of uridine and cytidine to UMP and CMP, playing a key role in pyrimidine salvage and nucleotide metabolism.
• The protein localizes predominantly to the cytoplasm and nucleus, consistent with its involvement in maintaining cellular nucleotide pools.
• UCKL1 is a phosphoprotein and undergoes ubiquitin-like conjugation, suggesting regulatory post-translational modifications.
• Ubiquitously expressed across human tissues, UCKL1 likely serves a fundamental housekeeping function.
• UniProt keywords include ATP-binding, kinase, transferase, and host-virus interaction, hinting at potential roles in viral replication.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1-200) of human UCKL1, which is conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blotting, expect a band at approximately 61 kDa; validate in your target species and relevant tissue lysates, as UCKL1 is ubiquitously expressed.
• For immunohistochemistry (paraffin), antigen retrieval optimization may be required; include appropriate positive and negative tissue controls.
• In ELISA, using purified recombinant UCKL1 as a coating antigen can help establish a standard curve and confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive provider of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. The anti-UCKL1 rabbit polyclonal antibody is part of our broad portfolio of nucleotide metabolism-related reagents, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We also specialize in sourcing rare-target raw materials, enabling you to accelerate assay development with a single, reliable partner.