Anti-UCHL5IP Rabbit Monoclonal Antibody for WB, ELISA - Q99871

Core Product Specifications and Parameters

Biological Background: HAUS7/UCHL5IP Function and Localization

• HAUS7 (also known as UCHL5IP, UIP1) is the HAUS augmin-like complex subunit 7, a component of the augmin complex critical for proper mitotic spindle formation and centrosome integrity.
• Key functions include contribution to mitotic spindle assembly, maintenance of centrosome integrity, and completion of cytokinesis.
• Encoded by the HAUS7 gene (Gene ID 55559) on the X chromosome, the protein is subject to alternative splicing.
• Subcellularly, HAUS7 localizes to the cytoplasm, cytoskeleton, centrosome, and mitotic spindle.
• Tissue expression is broad, detected in spleen, thymus, testis, ovary, small intestine, and colon, with highest levels in testis and ovary.
• Post-translational modifications include phosphorylation, and the protein features coiled-coil domains that mediate protein-protein interactions.
• Keywords from UniProt: Cell cycle, Cell division, Mitosis, Coiled coil, Phosphoprotein, indicating its essential role in cell division.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 150-285, a central region that may detect multiple isoforms; validate specificity in your target system.
• For Western blotting, expect a band near 40 kDa (calculated MW 39.8 kDa); use appropriate controls.
• In ELISA, consider employing the recombinant immunogen protein as a positive control to assess antibody performance.
• Optimize antibody concentration for each application; a typical starting dilution for WB might be 1:1000–1:5000, but this has not been experimentally validated for this product.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides comprehensive one-stop sourcing for IVD raw materials, including validated primary antibodies like this anti-HAUS7/UCHL5IP rabbit monoclonal antibody. With expertise in mitotic spindle and cell cycle targets, we serve diagnostic manufacturers and research organizations by offering antibody pairs, bulk ancillary reagents, and customized procurement solutions. Contact us to integrate this high-specificity antibody into your next assay development pipeline.