Anti-UCHL5 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y5K5

Core Product Specifications and Parameters

Biological Background: UCHL5 Function and Localization

• UCHL5, also known as ubiquitin carboxyl-terminal hydrolase isozyme L5, ubiquitin C-terminal hydrolase UCH37, or ubiquitin thioesterase L5, is a deubiquitinating enzyme encoded by the UCHL5 gene in humans.
• It functions as a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, playing a key role in the ubiquitin-proteasome system.
• UCHL5 is associated with the 19S regulatory subunit of the 26S proteasome, where it contributes to substrate deubiquitination prior to degradation.
• It serves as a putative regulatory component of the INO80 chromatin remodeling complex; however, it remains inactive within INO80 and is activated only upon transient interaction with the proteasome via ADRM1.
• Subcellular localization is both cytoplasmic and nuclear, consistent with roles in proteasomal degradation and chromatin-associated processes.
• The protein is subject to acetylation and alternative splicing, and is implicated in DNA damage response, DNA repair, recombination, transcription regulation, and the ubiquitin conjugation pathway, as reflected by its keyword annotations.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 1-328 of human UCHL5 (NP_001186190.1), covering the majority of the canonical isoform (329 residues). Due to the full-length nature of the immunogen, the antibody may recognize multiple UCHL5 isoforms or splice variants; validation in the relevant experimental system is recommended.
• For Western blotting, the expected molecular weight of UCHL5 is approximately 38 kDa. Ensure proper sample preparation and loading controls; consider using protease inhibitor cocktails to prevent target degradation.
• In immunohistochemistry (paraffin-embedded tissues, IHC-P), antigen retrieval conditions should be optimized, as the epitopes may be masked by formalin fixation. Titration of primary antibody concentration is advised to achieve specific staining.
• For immunofluorescence/immunocytochemistry (IF/ICC), choose fixation and permeabilization methods appropriate for nuclear and cytoplasmic detection. Use appropriate secondary antibodies with minimal cross-reactivity to host species.
• ELISA applications may require optimization of coating antigen and detection system. Consider pairing this polyclonal detection antibody with a monoclonal capture antibody for sandwich ELISA development.
• The antibody has reported cross-reactivity with human, mouse, and rat; when working with other species, verify sequence homology and perform a pilot experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop partner for IVD raw material sourcing, providing diagnostic manufacturers, laboratories, and research institutes with seamless access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. For researchers investigating the ubiquitin-proteasome pathway, chromatin remodeling, or DNA damage repair, CamelBio can support your project from concept to clinic with tailored technical services and supply solutions centered around key targets like UCHL5.