Anti-UCHL3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P15374

Core Product Specifications and Parameters

Biological Background: UCHL3 Function and Localization

• UCHL3 (Ubiquitin carboxyl-terminal hydrolase isozyme L3), also known as UCH-L3, is a deubiquitinating enzyme (DUB) belonging to the ubiquitin C-terminal hydrolase family. It functions as a thiol protease that controls cellular ubiquitin levels by processing ubiquitin precursors and ubiquitinated proteins. The enzyme exhibits substrate specificity for ubiquitin and NEDD8, with a preference for 'Lys-48'-linked ubiquitin chains and Arg/Lys at position P3''.
  Related references: PMID:19154770, PMID:21762696, PMID:22689415
• UCHL3 can also hydrolyze UBB(+1), a mutated form of ubiquitin implicated in neurodegenerative disorders (By similarity).
• It plays a role in DNA repair by mediating the deubiquitination of RAD51 in response to DNA damage, promoting the interaction between RAD51 and BRCA2 during homologous recombination.
  Related references: PMID:27941124
• The protein is localized predominantly in the cytoplasm.
• UCHL3 is highly expressed in heart, skeletal muscle, and testis, consistent with its roles in stress-response maintenance and cellular homeostasis.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword), and the three-dimensional structure of UCHL3 has been determined (3D-structure keyword).

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of UCHL3 is approximately 26 kDa. Consider using lysates from tissue with high expression (heart, skeletal muscle, testis) as positive controls.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval optimization may be necessary due to the intracellular cytoplasmic localization; validate in known positive tissues.
• The antibody is reported to cross-react with human, mouse, and rat UCHL3, making it suitable for studies across these species. Validate species-specific reactivity in your experimental system.
• As a rabbit monoclonal antibody, expect high batch-to-batch consistency. Store at -20°C upon receipt and avoid repeated freeze-thaw cycles to maintain activity.
• This antibody is recommended for ELISA applications; consider pairing with a detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for deubiquitinase research, offering this UCHL3 rabbit monoclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and sourcing support for rare targets. Our supply chain supports diagnostic manufacturers and labs from concept to clinic with reliable, high-quality reagents.