Anti-UCHL3 Polyclonal Antibody for WB, ELISA - P15374

Core Product Specifications and Parameters

Biological Background: UCHL3 Function and Localization

• UCHL3 (Ubiquitin carboxyl-terminal hydrolase isozyme L3), also known as ubiquitin thioesterase L3, is encoded by the UCHL3 gene in humans.
• UCHL3 is a thiol protease deubiquitinating enzyme (DUB) that hydrolyzes the C-terminal glycine of ubiquitin or NEDD8, controlling cellular ubiquitin pools through processing of ubiquitin precursors and ubiquitinated proteins. Related references: PMID:19154770 PMID:21762696 PMID:22689415
• The enzyme exhibits a 10-fold preference for Arg/Lys at position P3'' and preferentially cleaves 'Lys-48'-linked ubiquitin chains. Related references: PMID:19154770 PMID:21762696 PMID:22689415
• UCHL3 promotes DNA repair via homologous recombination by deubiquitinating RAD51 in response to DNA damage, thereby facilitating RAD51–BRCA2 interaction. Related references: PMID:27941124
• Additional functions include processing the neurodegeneration-associated UBB(+1) mutant ubiquitin, regulating ENaC-mediated apical membrane recycling, and enhancing insulin signaling via IGF1R/AKT/FOXO1 phosphorylation (By similarity).
• Highly expressed in heart, skeletal muscle, and testis; primarily localized in the cytoplasm.
• Classified as a phosphoprotein and member of the ubiquitin carboxyl-terminal hydrolase family, highlighting its role in the Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human UCHL3 protein (amino acids 1–220); in Western blotting, expect a specific band near 26 kDa under reducing conditions. Validate detection in your target sample type.
• Reported cross‑reactivity with mouse and rat UCHL3; however, we recommend confirming reactivity with your specific model systems before large‑scale experiments.
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimal working concentrations and buffer conditions should be empirically determined.
• Due to UCHL3’s cytoplasmic localization, use cytosolic fractions or mild lysis buffers to ensure efficient extraction of the native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one‑stop partner for IVD raw‑material sourcing, supporting diagnostic developers and research laboratories from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-UCHL3 polyclonal antibody complements our offerings for ubiquitin pathway research, with confirmed performance in WB and ELISA formats.