Anti-UBR5 Rabbit Polyclonal Antibody for WB and ELISA - O95071

Core Product Specifications and Parameters

Biological Background: UBR5 Function and Localization

• UBR5 (E3 ubiquitin-protein ligase UBR5) is a HECT domain-containing E3 ligase that acts as a ubiquitin chain elongator in cytoplasmic and nuclear protein quality control pathways. Related references: PMID:29033132, PMID:33208877, PMID:37478846
• It participates in the N-end rule pathway, recognizing type-1 N-degrons with positively charged N-terminal residues (Arg, Lys, His) and targeting substrates for proteasomal degradation.
• Together with UBR4, UBR5 catalyzes assembly of heterotypic Lys-11/Lys-48-linked branched ubiquitin chains on aggregated proteins, enabling p97/VCP-mediated extraction and proteasomal degradation. Related references: PMID:29033132
• UBR5 collaborates with ITCH to mediate Lys-48/Lys-63-branched ubiquitination of TXNIP, directing it for degradation. Related references: PMID:29378950
• In the nucleus, UBR5 ubiquitinates unpaired transcription factors (e.g., MYC, SUPT4H1, SUPT5H, CDC20, MCRS1) and ligand-bound nuclear receptors (e.g., ESR1, NR1H3, NR3C1, PGR, RARA, RXRA, VDR) not associated with coactivators, ensuring proteolytic clearance of unassembled regulators. Related references: PMID:33208877, PMID:37478846, PMID:37478862
• UBR5 suppresses excessive DNA damage signaling by counteracting RNF168-mediated histone H2A/H2AX ubiquitination and regulating TopBP1, thus preventing over-spreading of ubiquitinated chromatin. Related references: PMID:22884692, PMID:11714696
• It positively regulates Wnt signaling by stabilizing CTNNB1 and mediating Lys-48-linked ubiquitination and degradation of the transcriptional corepressor TLE3. Related references: PMID:21118991, PMID:28689657
• UBR5 promotes disassembly of the mitotic checkpoint complex by ubiquitinating BUB1B, BUB3, and CDC20, facilitating APC/C activation. Related references: PMID:35217622
• Widely expressed, with highest levels in testis, brain, pituitary, and kidney; localizes to both nucleus and cytoplasm.

Experimental Guidance and Technical Tips

• The immunogen (aa 550-760 of human UBR5) targets a central region of the ~309 kDa protein; consider using low-percentage SDS-PAGE gels and prolonged transfer for Western blot detection.
• For ELISA applications, the polyclonal nature may be suitable for capture or detection of recombinant UBR5 fragments; validate pairing with a compatible detection antibody.
• Validate antibody reactivity in the intended human sample type (e.g., cell lysates, tissue extracts) and include appropriate positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio provides diagnostic developers and researchers with reliable access to UBR5 antibodies and a broad portfolio of ubiquitin-proteasome pathway reagents. We streamline procurement of validated polyclonal antibodies, optimized matched pairs, and ancillary raw materials—helping you accelerate assay development from concept to clinic. Whether you need bulk supply or rare target sourcing, CamelBio is your bridge to scalable, quality-driven solutions.