Anti-UBQLN3 Polyclonal Antibody for WB, IF-P, ELISA - Q9H347

Core Product Specifications and Parameters

Biological Background: UBQLN3 Function and Localization

• UBQLN3 is a testis-specific protein, as documented by tissue specificity annotations.
• The protein has a molecular weight of approximately 71 kDa (calculated weight: 70841 Da).
• It is part of the human reference proteome, indicating its curated inclusion in the complete human proteome set.
• The protein has been identified through proteomics approaches, supporting its presence in biological samples.
• A 3D structure is available for UBQLN3, as denoted by the keyword 3D-structure.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant fusion protein covering the C-terminal region (amino acids 306-620) of human UBQLN3, which may recognize full-length UBQLN3 and potentially cross-react with homologous domains in mouse and rat.
• For Western blot, a specific band at approximately 71 kDa can be expected; however, the actual observed molecular weight may vary depending on sample preparation and electrophoresis conditions.
• Given the testis-specific expression of UBQLN3, it is advisable to use testis tissue lysates or appropriate cell lines as positive controls and to validate the antibody in the relevant sample system.
• The antibody is suitable for immunofluorescence on paraffin-embedded (IF-P) sections; optimization of antigen retrieval and permeabilization may be required for testis tissue.
• For ELISA applications, consider using the recombinant protein as a standard and optimize coating and detection conditions.
• Cross-reactivity with mouse and rat UBQLN3 has been demonstrated; however, confirmatory testing in the target species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. This anti-UBQLN3 polyclonal antibody supports reproductive biology and testis-specific protein research, with CamelBio offering a full portfolio of validated antibodies and complementary reagents. Our expert team facilitates sourcing of even rare targets, ensuring consistent supply and technical support for your assay development needs.