Anti-UBQLN1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UMX0

Core Product Specifications and Parameters

Biological Background: UBQLN1 Function and Localization

• Ubiquilin-1 is a 62.5 kDa protein encoded by the UBQLN1 gene, containing an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. These domains mediate interactions with proteasomal subunits and polyubiquitinated substrates, respectively, to facilitate proteasomal degradation (PubMed:15147878). Related references: PMID:15147878
• It participates in endoplasmic reticulum-associated protein degradation (ERAD) by forming a link between polyubiquitinated ERAD substrates and the proteasome through interactions with ER-localized proteins UBXN4, VCP, and HERPUD1 (PubMed:18307982, PubMed:19822669). Related references: PMID:18307982, PMID:19822669
• Ubiquilin-1 is required for macroautophagy: it promotes the conversion of LC3-I to LC3-II and aids in autophagosome-lysosome fusion, thereby facilitating autophagic degradation (PubMed:19148225, PubMed:20529957, PubMed:23459205). Related references: PMID:19148225, PMID:20529957, PMID:23459205
• It negatively regulates innate immune signaling by targeting TICAM1 for autophagic degradation (PubMed:21695056) and promotes ubiquitination and lysosomal degradation of ORAI1, thereby down-regulating store-operated Ca2+ entry (PubMed:23307288). Related references: PMID:21695056, PMID:23307288
• During ER stress, Ubiquilin-1 attenuates the unfolded protein response (UPR) by reducing the induction of UPR target genes (PubMed:18953672) and targets PSEN1 to aggresomes for autophagic clearance (PubMed:21143716). Related references: PMID:18953672, PMID:21143716
• Ubiquilin-1 is ubiquitously expressed, with high levels in brain, heart, placenta, pancreas, lung, liver, skeletal muscle, and kidney. In the brain, it localizes to neurons and pathological lesions such as neurofibrillary tangles and Lewy bodies (PubMed:18953672). Related references: PMID:18953672

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 480–540 near the C-terminus of human UBQLN1, encompassing part of the UBA domain. The antibody is expected to recognize full-length UBQLN1 and may detect isoforms containing this region.
• For Western blotting, the predicted molecular weight is approximately 63 kDa. Use appropriate blocking and wash conditions, and validate with positive and negative controls. Cross-reactivity with mouse and rat allows for testing in common model systems, but we recommend confirming specificity in your experimental context.
• For ELISA, consider using recombinant UBQLN1 or the immunogen peptide as a coating antigen. Optimize coating concentration and antibody dilution for best signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot variability may occur. It is advisable to titrate each new lot and validate performance in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For ubiquitin-proteasome, autophagy, and ER stress research, we offer validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to support your studies on UBQLN1 and related pathways. Contact us to discuss bulk sourcing or custom development to meet your diagnostic or research needs.