Anti-UBL5 Rabbit Polyclonal Antibody for WB and ELISA - Q9BZL1

Core Product Specifications and Parameters

Biological Background: Ubiquitin-like protein 5 Function and Localization

• Ubiquitin-like protein 5 (UBL5), also known as HUB1, is a small 73-amino acid protein belonging to the ubiquitin-like family.
• UBL5 plays a critical role in pre-mRNA splicing by maintaining spliceosome integrity and associating with spliceosomal proteins, which is essential for proper gene expression (PubMed:25092792). Related references: PMID:25092792
• It promotes the functional integrity of the Fanconi anemia DNA repair pathway by interacting with FANCI and mediating FANCI homodimer formation (PubMed:25862789). Related references: PMID:25862789
• UBL5 has a protective role against endoplasmic reticulum (ER) stress-induced apoptosis, indicating involvement in cellular stress responses (PubMed:37315790). Related references: PMID:37315790
• Subcellularly, UBL5 localizes to the cytoplasm, nucleus, and Cajal bodies, reflecting its dynamic roles in RNA processing and DNA repair.
• It is ubiquitously expressed, with highest levels in heart, skeletal muscle, kidney, liver, iris, and lymphoblasts.
• Keywords from UniProt include: 3D-structure, Cytoplasm, Nucleus, Proteomics identification, Reference proteome, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length UBL5 protein (residues 1-73), suggesting the antibody may detect both endogenous and recombinant UBL5; however, users should validate in their specific sample system.
• For Western blot, the predicted molecular weight is approximately 9 kDa; consider using a high-percentage gel or specialized transfer protocol for small proteins.
• In ELISA applications, this polyclonal antibody can be used as capture or detection reagent; optimum working dilutions should be determined empirically.
• Cross-reactivity is reported for human; if using other species, verify sequence homology and perform cross-reactivity validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop supplier of IVD raw materials, supporting diagnostic manufacturers and research labs with high-quality reagents. For UBL5-related studies in pre-mRNA splicing, DNA repair, and stress response pathways, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our expert team also assists in sourcing rare targets, ensuring you have the tools needed from concept to clinic.