Anti-UBL4A Rabbit Polyclonal Antibody for WB, IP, ELISA - P11441

Core Product Specifications and Parameters

Biological Background: UBL4A Function and Localization

• Ubiquitin-like protein 4A (UBL4A), also known as GDX, GET5, MDY2, TMA24, and DX254E, is a component of the BAG6/BAT3 protein quality control complex.
• As part of the BAG6/BAT3 complex, UBL4A maintains misfolded and hydrophobic patches-containing proteins in a soluble state and facilitates their delivery to the endoplasmic reticulum or proteasome (PubMed:20676083, PubMed:21636303, PubMed:21743475). Related references: PMID:20676083 PMID:21636303 PMID:21743475
• The complex mediates post-translational delivery of tail-anchored/type II transmembrane proteins to the ER membrane, interacting with the nascent transmembrane region at the ribosome (PubMed:20676083, PubMed:25535373, PubMed:28104892). Related references: PMID:20676083 PMID:25535373 PMID:28104892
• UBL4A is involved in sorting mislocalized secretory pathway proteins, either targeting them to the proteasome for degradation or redirecting them to the ER (PubMed:21743475). Related references: PMID:21743475
• It contributes to ER-associated degradation (ERAD) by maintaining retrotranslocated ER proteins in an unfolded, soluble state for proteasomal delivery (PubMed:21636303). Related references: PMID:21636303
• UBL4A localizes to the cytoplasm, cytosol, and nucleus.
• It is annotated as a phosphoprotein and can undergo ubiquitin-like conjugation; it is also associated with an isopeptide bond.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length protein (aa 1-157), enabling detection of both endogenous UBL4A and overexpressed constructs.
• For Western blot, a band near 18 kDa is expected; validate specificity using human, mouse, or rat lysates and consider loading controls.
• Polyclonal antibodies may require titration to minimize non-specific background; optimization of blocking and primary antibody dilution is advised.
• In immunoprecipitation, be aware that UBL4A is part of a dynamic complex (BAG6/BAT3) and adjust lysis/wash conditions to preserve native interactions or denature as needed.
• ELISA protocols should be optimized for coating antigen and antibody concentration, as the antibody is raised against recombinant protein but not pre-validated for specific sandwich pairs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw materials, offering reliable access to high-quality antibodies like this anti-UBL4A polyclonal antibody. Whether you need validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, or sourcing for rare targets, CamelBio supports your diagnostic development from concept to clinic. Our portfolio covers key research areas including protein quality control and cellular trafficking, ensuring your assay development is powered by consistent, high-performance raw materials.