Anti-UBL3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95164

Core Product Specifications and Parameters

Biological Background: UBL3 Function and Localization

• Ubiquitin-like protein 3 (UBL3) is a 117-amino-acid protein belonging to the ubiquitin-like family, sharing structural homology with ubiquitin. It is also known as membrane-anchored ubiquitin-fold protein (MUB) or protein HCG-1.
• The protein is anchored to the cell membrane via post-translational lipid modifications, including palmitoylation and prenylation, which are essential for its subcellular localization.
• UBL3 is a lipoprotein that stably associates with the cytoplasmic face of the cell membrane, where it may participate in membrane-related signaling or trafficking processes.
• It is ubiquitously expressed across human tissues, suggesting a fundamental cellular role.
• The protein undergoes methylation, as indicated by proteomics identification, which may regulate its activity or interactions.
• Structural studies have resolved the 3D structure of UBL3, revealing a canonical ubiquitin fold despite its membrane-anchored nature.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human UBL3 (aa 1-117), which is highly conserved in mouse and rat, likely contributing to the observed cross-reactivity. Validate specificity in your species of interest.
• For Western blotting, a band around 13 kDa is expected, but migration may shift due to lipidation. Use appropriate membrane blocking and detection conditions.
• In IHC-P experiments, anticipate membrane-localized staining. Optimization of antigen retrieval and antibody dilution is recommended.
• This antibody is suitable for ELISA, allowing detection and quantification of UBL3 in various sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-UBL3 rabbit polyclonal antibody as part of our extensive IVD raw material portfolio, ideal for studies on membrane-anchored ubiquitin-like proteins. As your one-stop sourcing partner, we offer validated antibodies, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development. Contact us for comprehensive technical support and supply chain solutions tailored to diagnostic and research needs.