Anti-Ubiquitin Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P0CG47

Core Product Specifications and Parameters

Biological Background: Ubiquitin (Polyubiquitin-B) Function and Localization

• Ubiquitin, encoded by the UBB gene, is a highly conserved 76-amino acid protein that exists either free (unanchored) or covalently attached to target proteins via an isopeptide bond. It can form polyubiquitin chains linked through different lysine residues or linear chains via the initiator methionine.
• Polyubiquitin chains serve diverse functions: Lys48-linked chains target proteins for proteasomal degradation; Lys63-linked chains are involved in endocytosis, DNA repair, and NF-kappa-B signaling; other linkages (Lys6, Lys11, Lys29, Lys33) regulate processes such as DNA repair, ERAD, cell cycle, proteotoxic stress response, and kinase modification.
• The protein localizes to the cytoplasm, nucleus, and mitochondrion outer membrane, reflecting its wide-ranging roles in cellular homeostasis and signaling.
• Ubiquitin is subject to various post-translational modifications including phosphorylation and ADP-ribosylation, and it frequently forms isopeptide bonds during conjugation.
• As a member of the ubiquitin protein family, UBB represents a polyubiquitin precursor that is processed into mature ubiquitin monomers, ensuring a steady supply for cellular needs.
• Ubiquitin conjugation generally targets lysine residues on substrates, though rare conjugation to cysteine or serine residues has been observed, expanding the scope of ubiquitin signaling.

Experimental Guidance and Technical Tips

• The immunogen comprises human Ubiquitin amino acids 1-100, which covers the full-length mature protein, so the antibody may recognize total ubiquitin. Consider using appropriate controls to distinguish unanchored ubiquitin from conjugated forms in your assays.
• For Western blotting (WB), a band around 26 kDa may correspond to polyubiquitin-B precursor, but individual ubiquitin monomers (~8.5 kDa) conjugated to targets will appear as higher molecular weight smears. Optimize sample preparation and detection conditions to resolve polyubiquitinated species.
• In IHC-P and IF/ICC, verify specificity by comparing staining patterns in known positive and negative tissues/cell lines, and include a blocking peptide competition assay if available.
• For ELISA applications, ensure that the recombinant immunogen or purified ubiquitin is used as a standard to validate sensitivity and linearity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers Ubiquitin Rabbit mAb as a reliable tool for studying ubiquitin-mediated proteolysis, DNA repair, and cellular signaling pathways. As your one-stop IVD raw-material sourcing partner, we provide validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic and research programs from concept to clinic.