Anti-Ubiquitin Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P0CG47

Core Product Specifications and Parameters

Biological Background: Polyubiquitin-B Function and Localization

• Polyubiquitin-B, encoded by the UBB gene, is a ubiquitin precursor that yields multiple ubiquitin units upon processing.
• Ubiquitin exists either covalently attached to target proteins (monoubiquitin or polyubiquitin chains) or free (unanchored); polyubiquitin chains are linked via different Lys residues (e.g., Lys-6, Lys-11, Lys-29, Lys-33, Lys-48, Lys-63) or via the initiator Met, each with distinct cellular functions.
• Lys-48-linked polyubiquitin chains target proteins for proteasomal degradation, while Lys-63-linked chains mediate non-degradative signaling such as endocytosis, DNA-damage response, and NF-kappa-B activation.
• Other linkage types include: Lys-6 (DNA repair), Lys-11 (ERAD and cell-cycle regulation), Lys-29 (proteotoxic stress response and cell cycle), Lys-33 (kinase modification), and linear chains (cell signaling).
• Unanchored free polyubiquitin also functions in protein kinase activation and signaling pathways.
• Ubiquitin is predominantly localized in the cytoplasm and nucleus, and is also found on the mitochondrial outer membrane.
• Post-translational modifications of ubiquitin include phosphorylation and ADP-ribosylation.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant fusion protein covering full-length human ubiquitin (aa 1-228), which includes all key residues for recognition of both monomeric and polyubiquitin forms.
• For Western blot (WB), the predicted molecular weight is 26 kDa; however, due to ubiquitin conjugation to various targets, a range of bands at higher molecular weights may be observed, reflecting polyubiquitinated proteins.
• When performing immunohistochemistry (IHC-P), consider using a standard antigen retrieval method and validate staining specificity with appropriate positive and negative control tissues.
• The antibody’s reactivity with a wide range of species (predicted) suggests conserved epitopes; verify cross-reactivity in the specific experimental system if using species other than human, mouse, or rat.
• For ELISA applications, the recombinant immunogen can serve as a positive control to establish assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and IVD developers with one-stop access to high-quality raw materials, including validated monoclonal and polyclonal antibodies, antigen proteins, and ancillary reagents. For ubiquitin-proteasome pathway research, CamelBio offers this anti-ubiquitin rabbit mAb alongside comprehensive sourcing support for rare targets, bulk custom orders, and technical consulting—streamlining your transition from concept to clinic.