Anti-Ubiquilin-2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UHD9

Core Product Specifications and Parameters

Biological Background: Ubiquilin-2 Function and Localization

• Ubiquilin-2 (UBQLN2), also known as DSK2 homolog, PLIC-2, CHAP1, and N4BP4, is a ubiquitin-like protein encoded by the UBQLN2 gene.
• It plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy, and endoplasmic reticulum-associated protein degradation (ERAD). Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:10983987). Related references: PMID:10983987
• UBQLN2 participates in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:18307982, PubMed:24215460). Related references: PMID:18307982, PMID:24215460
• Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957). Related references: PMID:19148225, PMID:20529957
• Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs) (PubMed:18199683). Related references: PMID:18199683
• Subcellularly, UBQLN2 localizes to the cytoplasm, nucleus, membrane, and cytoplasmic vesicles including autophagosomes.
• Post-translational modifications include acetylation; mutations in UBQLN2 are associated with amyotrophic lateral sclerosis (ALS) and neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 1-150 amino acids of human UBQLN2; consider epitope accessibility and potential isoform-specific recognition in your assay design.
• For Western blot, validate antibody specificity using relevant positive controls (e.g., human or mouse cell lysates) and consider siRNA knockdown or CRISPR knock-out controls to confirm target band identity.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization protocols; a nuclear/cytoplasmic staining pattern is expected.
• This antibody cross-reacts with mouse, allowing its use in mouse tissue or cell models; verify cross-reactivity in your specific system.
• As a rabbit polyclonal, it is compatible with standard anti-rabbit secondary detection reagents for colorimetric, fluorescent, or ELISA applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinical application. With a focus on critical biological pathways like protein degradation and autophagy, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expert team assists in rare target raw-material sourcing to accelerate your assay development and ensure reliable supply chain continuity.