Anti-UBIAD1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y5Z9

Core Product Specifications and Parameters

Biological Background: UBIAD1 Function and Localization

• UBIAD1 (UbiA prenyltransferase domain-containing protein 1), also known as transitional epithelial response protein 1 (TERE1), is a multi-pass membrane protein that belongs to the UbiA prenyltransferase family.
• It functions as a prenyltransferase that catalyzes the biosynthesis of menaquinone-4 (MK-4) and coenzyme Q10 (ubiquinone). Related references: PMID:20953171, PMID:23374346
• MK-4 is a vitamin K2 isoform essential for endothelial cell development, and UBIAD1 mediates its formation from phylloquinone (PK) via menadione (K3). Related references: PMID:20953171
• As a coenzyme Q10 biosynthetic enzyme in the Golgi membrane, UBIAD1 protects cardiovascular tissues from NOS3/eNOS-dependent oxidative stress. Related references: PMID:23374346
• The protein localizes to multiple organelles including the endoplasmic reticulum membrane, Golgi apparatus membrane, mitochondrion membrane, cytoplasm, and nucleus.
• UBIAD1 is ubiquitously expressed across tissues.
• Post-translational modifications include acetylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide within the N-terminal region (amino acids 1-100) of human UBIAD1, suggesting the antibody may detect the full-length protein and potentially N-terminal fragments. Validate specificity in your experimental system.
• For Western blot (WB), the predicted molecular weight is approximately 37 kDa (observed band may vary due to post-translational modifications); include appropriate positive and negative controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider testing different antigen retrieval methods and antibody dilutions to optimize staining.
• ELISA applications may benefit from pairing with a validated capture/detection antibody; verify cross-reactivity with mouse samples if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This UBIAD1 antibody supports studies in vitamin K2 and coenzyme Q10 biosynthesis, cardiovascular biology, and related disorders. We offer validated antibody pairs, optimized polyclonal antibodies like this one, bulk ancillary reagents, and assist with rare target raw-material sourcing—covering every stage from concept to clinic.