Anti-UBE4B Monoclonal Antibody for WB, ELISA - O95155

Core Product Specifications and Parameters

Biological Background: UBE4B Function and Localization

• UBE4B (Ubiquitin Conjugation Factor E4 B) is a multi-domain protein functioning as both an E3 and E4 ubiquitin ligase in the ubiquitin-proteasome system.
• It catalyzes the transfer of ubiquitin from E2 enzymes to substrates and can assemble polyubiquitin chains on pre-ubiquitinated targets, facilitating proteasomal degradation.
• In striated muscle, UBE4B cooperates with STUB1 and VCP/p97 to ubiquitinate the myosin chaperone UNC45B, a step critical for myosin assembly regulation. Related references: PMID:17369820
• The protein localizes to both the cytoplasm and the nucleus, consistent with its roles in diverse cellular processes.
• UBE4B is predominantly expressed in ovary, testis, heart, and skeletal muscle at the transcript level, and it is detected in differentiated myotubes at the protein level. Expression is low in colon, thymus, and peripheral blood leukocytes, and almost undetectable in lung and spleen. Related references: PMID:17369820 PMID:11802788
• Post-translational modifications include phosphorylation and acetylation; alternative splicing generates multiple isoforms, potentially modulating its activity and interactions.
• The RING-type zinc finger domain is essential for its E3 ligase activity, and the protein's three-dimensional structure has been studied to understand substrate recognition mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 within the N-terminus of human UBE4B. Consider verifying antibody specificity by western blot using lysates from tissues with high UBE4B expression, such as heart or skeletal muscle.
• For WB applications, a band around 146 kDa is expected. Due to the large protein size, ensure adequate protein transfer conditions (e.g., sufficient transfer time) and use appropriate molecular weight markers.
• This antibody cross-reacts with mouse and rat UBE4B; pilot experiments in these species should include appropriate controls to confirm reactivity and specificity.
• ELISA protocols may require optimization of coating buffer and antibody concentration; the antibody can be used for either direct antigen detection or as a capture/detection pair component.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with critical reagents for ubiquitin-proteasome pathway studies. From validated monoclonal antibodies like this anti-UBE4B clone to bulk ancillary reagents and custom rare-target sourcing, we offer comprehensive support to accelerate your assay development from concept to clinic. Leverage CamelBio’s integrated supply chain for efficient access to high-performance raw materials.