Anti-UBE4A Monoclonal Antibody for WB, ELISA - Q14139

Core Product Specifications and Parameters

Biological Background: UBE4A Function and Localization

• UBE4A is a ubiquitin conjugation factor E4 A, also identified as a RING-type E3 ubiquitin transferase. It is alternatively named E4, UFD2, UBOX2, or NEDHMS.
• It functions as an E3 ubiquitin-protein ligase, likely in conjunction with specific E1 and E2 enzymes. Additionally, it may act as an E4 ligase, assembling polyubiquitin chains on substrates already ubiquitinated by another E3 ligase.
• The enzyme specifically mediates Lys-48-linked polyubiquitination, a signal for proteasomal degradation.
• Subcellular localization is primarily cytoplasmic.
• UBE4A is characterized by its U-box domain (aliases UBOX2), classifying it as a U-box E3 ubiquitin ligase.
• It plays a role in the ubiquitin-conjugating pathway, as indicated by its functional annotations.
• Genetic variants in UBE4A are associated with intellectual disability, highlighting its importance in neurodevelopment.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to the N-terminal region (amino acids 1–100) of human UBE4A. Consider validating reactivity with recombinant full-length protein if non-specific binding is suspected.
• For Western blot, a band around 123 kDa is expected. Use appropriate molecular weight markers and consider the possibility of post-translational modifications or isoforms that may shift mobility.
• ELISA applications may require optimization of coating conditions and antibody concentrations; cross-reactivity with other species or related proteins should be assessed in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. As a sourcing bridge, we offer validated antibody pairs, bulk reagents, and optimized antibodies for ubiquitin pathway targets like UBE4A. Our comprehensive supply chain supports your assay development from concept to clinic.