Anti-UBE2V2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15819

Core Product Specifications and Parameters

Biological Background: UBE2V2 Function and Localization

• UBE2V2 (also known as MMS2, UEV2, EDPF1, DDVIT1) is a ubiquitin-conjugating enzyme E2 variant that lacks intrinsic ubiquitin ligase activity.
• It forms a heterodimer with UBE2N (Ubc13) to catalyze the assembly of Lys-63-linked polyubiquitin chains, which signal non-proteasomal processes.
• The UBE2V2-UBE2N complex mediates transcriptional activation and plays a role in regulating cell cycle progression and cellular differentiation.
• UBE2V2 participates in error-free DNA damage repair pathways, promoting cell survival after genotoxic stress.
• It is expressed in placenta, colon, liver, and skin, with very low levels detected in most other tissues.
• Key features include acetylation as a post-translational modification and a solved 3D structure; it is part of the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• For western blot validation, consider that UBE2V2 has a predicted molecular weight of ~16 kDa; adjust SDS-PAGE gel percentage and transfer conditions accordingly.
• The immunogen (aa 1-145) targets the N-terminal region; verify reactivity with the expected band in lysates from human, mouse, or rat tissues.
• When performing IHC-P, optimize antigen retrieval and detection protocols for your specific tissue type, as expression levels may vary.
• Cross-reactivity with mouse and rat is indicated; validate performance in the relevant species if your study requires interspecies comparisons.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a complete range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For ubiquitination and DNA repair targets like UBE2V2, CamelBio streamlines your development with reliable sourcing solutions, from concept to clinic. Contact us to explore how we can support your next project.