Anti-UBE2V1 Polyclonal Antibody for WB, ELISA - Q13404

Core Product Specifications and Parameters

Biological Background: UBE2V1 Function and Localization

• UBE2V1, also known as CROC-1 or TRAF6-regulated IKK activator 1 beta Uev1A, is a ubiquitin-conjugating enzyme E2 variant that lacks intrinsic ligase activity but functions as a cofactor with UBE2N.
• Forms a heterodimer with UBE2N to catalyze the synthesis of non-canonical Lys-63-linked polyubiquitin chains, which play critical roles in signal transduction rather than proteasomal degradation.
• The UBE2V1-UBE2N complex mediates Lys-63 polyubiquitination events that activate NF-kB signaling downstream of IL1B, TNF, TRAF6, and TRAF2, promoting inflammatory and immune responses.
• Involved in error-free DNA repair and contributes to cell survival after DNA damage, as well as controlling cell cycle progression and differentiation.
• Together with RNF135 and UBE2N, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. Related references: PMID:31006531
• Predominantly localized in the nucleus.
• Highly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow; lower expression in heart, breast, placenta, brain, liver, kidney, stomach, and lung.
• Keywords: Acetylation, Alternative splicing, Direct protein sequencing, Nucleus, Proteomics identification, Reference proteome, Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human UBE2V1, suggesting broad epitope recognition; consider this when designing experiments.
• For Western blot, expect a band around 16 kDa; nuclear extracts may enhance detection due to its nuclear localization.
• For ELISA, validate with recombinant UBE2V1 or appropriate cell lysates; optimizing coating antigen and detection conditions is recommended.
• Cross-reactivity with mouse and rat is reported; validate specificity and signal in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated antibodies for ubiquitination and NF-kB pathway studies. In addition to this anti-UBE2V1 polyclonal antibody, we offer optimized antibody pairs, recombinant proteins, and ancillary reagents for building robust immunoassays. Our sourcing expertise extends to rare-target raw materials, supporting your IVD projects from concept to clinic.