Anti-UBE2T Rabbit Polyclonal Antibody for WB, ELISA - Q9NPD8

Core Product Specifications and Parameters

Biological Background: UBE2T Function and Localization

• UBE2T (Ubiquitin-conjugating enzyme E2 T), also known as FANCT, PIG50, or HSPC150, is a member of the ubiquitin-conjugating enzyme family that functions as an E2 enzyme in the ubiquitination cascade.
• It is a key component of the Fanconi anemia DNA repair pathway; it specifically catalyzes the monoubiquitination of FANCD2 and FANCI by acting as the E2 partner for the E3 ligase FANCL, which is critical for resolving DNA interstrand crosslinks. Related references: PMID:16916645, PMID:17938197, PMID:19111657
• It also mediates ubiquitination and degradation of the tumor suppressor BRCA1, linking UBE2T to homologous recombination repair and breast cancer susceptibility. Related references: PMID:19887602
• In vitro, UBE2T can build polyubiquitin chains using all seven lysine residues of ubiquitin, with a preference for Lys-11, Lys-27, Lys-48, and Lys-63 linkages, indicating versatile chain specificity. Related references: PMID:20061386
• Subcellular localization is primarily nuclear, where it supports DNA damage signaling and repair processes.
• The protein undergoes phosphorylation and is identified as a phosphoprotein; its association with Fanconi anemia highlights its clinical relevance in genomic stability disorders.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human UBE2T (amino acids 1-197, NP_054895.1), which suggests good epitope accessibility for recognizing endogenous protein across species (human, mouse, rat).
• For Western blotting, consider using a dilution series to determine the optimal antibody concentration, and ensure efficient nuclear protein extraction given UBE2T's nuclear localization.
• In ELISA, the antibody may be applied as a capture or detection reagent; validate pairing with suitable detection antibodies or antigens in your specific assay format.
• For DNA damage studies, induce crosslinking agents such as mitomycin C to monitor UBE2T-dependent monoubiquitination events.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD manufacturers and research laboratories, offering comprehensive raw material solutions from concept to clinical application. This anti-UBE2T polyclonal antibody represents our ability to deliver high-quality, validated reagents for DNA repair and ubiquitination research. Beyond antibodies, we supply recombinant proteins, optimized antibody pairs, and bulk ancillary materials, all backed by technical and regulatory support to accelerate your assay development.