Anti-UBE2S Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16763

Core Product Specifications and Parameters

Biological Background: UBE2S Function and Localization

• Ubiquitin-conjugating enzyme E2 S (UBE2S, also known as E2-EPF or UBE2S) is a member of the ubiquitin-conjugating enzyme family that accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to substrates.
• Plays an essential role in cell cycle regulation as a component of the anaphase-promoting complex/cyclosome (APC/C), where it specifically elongates 'Lys-11'-linked polyubiquitin chains initiated by UBE2C, enhancing the degradation of APC/C substrates and promoting mitotic exit. Related references: PMID:19820702 PMID:19822757 PMID:27259151
• Also mediates ubiquitination and subsequent degradation of VHL, leading to accumulation of HIF1A under certain conditions. Related references: PMID:16819549
• In vitro, UBE2S can promote polyubiquitination using all seven ubiquitin Lys residues except 'Lys-48', demonstrating its chain-linkage specificity. Related references: PMID:20061386 PMID:20622874
• The protein is subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity during the cell cycle.
• Listed keywords for UBE2S include: 3D-structure, Acetylation, Cell cycle, Cell division, Phosphoprotein, Proteomics identification, Reference proteome, Transferase, Ubl conjugation, Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is approximately 24 kDa. Optimize blocking and antibody dilution using a relevant cell lysate known to express UBE2S.
• In immunofluorescence/immunocytochemistry (IF/ICC), validate the antibody with appropriate fixation/permeabilization protocols and include controls for subcellular localization, as UBE2S is expected to show nuclear and cytoplasmic distribution.
• For ELISA, consider pairing with a validated capture or detection antibody after confirming specificity and sensitivity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-UBE2S polyclonal antibody supports research into ubiquitin-proteasome pathways and mitotic regulation. Complement your workflow with CamelBio’s validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials.