Anti-UBE2R2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q712K3

Core Product Specifications and Parameters

Biological Background: UBE2R2 Function and Localization

• UBE2R2 (Ubiquitin-conjugating enzyme E2 R2) is also known as CDC34B, UBC3B, and E2-CDC34B; it is a member of the ubiquitin-conjugating enzyme E2 family.
• As an E2 enzyme, UBE2R2 accepts ubiquitin from an E1 activating enzyme and catalyzes its covalent attachment to lysine residues on target proteins via E3 ligase complexes, mediating both monoubiquitination and Lys-48-linked polyubiquitination. Related references: PMID:12037680 PMID:20061386 PMID:38326650
• It functions in collaboration with various Cul1-RING and Cul2-RING E3 ubiquitin ligase complexes. Related references: PMID:38326650
• UBE2R2 may play a role in the degradation of katenin. Related references: PMID:12037680
• According to UniProt keywords, UBE2R2 is a phosphoprotein, suggesting regulation by post-translational modification.
• It is annotated under the “Ubl conjugation pathway” keyword, highlighting its central role in protein ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers nearly the full-length human UBE2R2 protein (amino acids 1–238), which may provide broad epitope recognition; consider testing in your specific assay conditions.
• For Western blot (WB), expect a band at approximately 27 kDa; optimize primary antibody dilution and incubation conditions for your sample type.
• For immunofluorescence/immunocytochemistry (IF/ICC), appropriate fixation and permeabilization protocols should be optimized to ensure accurate detection.
• Because cross-reactivity with mouse and rat is indicated, this antibody can potentially be used for these species; however, independent validation in the relevant model system is recommended.
• ELISA applicability suggests suitability for immunometric assays; consider checkerboard titration to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, connecting diagnostic developers with high-quality antibodies and reagents. This UBE2R2 polyclonal antibody supports research in the ubiquitin-proteasome pathway, and our team can assist with additional sourcing needs—from validated antibody pairs and bulk reagents to rare target raw materials—helping streamline your project from concept to clinic.