Anti-UBE2Q2 Polyclonal Antibody for WB, ELISA - Q8WVN8

Core Product Specifications and Parameters

Biological Background: UBE2Q2 Function and Localization

• UBE2Q2 (Ubiquitin-conjugating enzyme E2 Q2), also known as ubiquitin carrier protein Q2 or ubiquitin-protein ligase Q2, is a member of the ubiquitin-conjugating enzyme family.
• It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins, specifically mediating 'Lys-48'-linked polyubiquitination in vitro.
• UBE2Q2 is localized in the cytoplasm.
• Tissue specificity shows detection in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium.
• The protein is subject to alternative splicing, potentially producing multiple isoforms.
• Keywords from UniProt include: 3D-structure, Alternative splicing, Cytoplasm, Proteomics identification, Reference proteome, Transferase, Ubl conjugation, Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 1-150 of human UBE2Q2, which covers the N-terminal region. The antibody is expected to recognize epitopes within this region.
• For Western blotting (WB), UBE2Q2 has a predicted molecular weight of approximately 43 kDa (per supplier) and 42.8 kDa from UniProt. Include appropriate molecular weight markers and validate the detected band size in your specific sample system.
• This antibody is a rabbit polyclonal, so multiple epitopes may be recognized, potentially increasing sensitivity but also requiring careful validation for specificity in each application.
• Cross-reactivity is reported for human and mouse; however, for other species, perform sequence alignment of the immunogen region and validate experimentally.
• For ELISA, consider the antibody's reactivity with native and denatured protein forms, and optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic manufacturers developing ubiquitin pathway assays. As a dedicated IVD raw-material partner, CamelBio supports your project from concept to clinic—whether you need this UBE2Q2 polyclonal antibody or require sourcing assistance for rare targets in cancer research. Let us bridge your procurement needs with reliable, scalable, and regulation-ready supply.