Anti-UBE2O Rabbit Polyclonal Antibody [KO Validated] for WB, IF/ICC, IP, ELISA - Q9C0C9

Core Product Specifications and Parameters

Biological Background: UBE2O Function and Localization

• UBE2O (Ubiquitin-conjugating enzyme E2 O) is also known as E2-230K or 2O, a large 141 kDa E2/E3 hybrid ubiquitin-protein ligase.
• It displays both E2 and E3 ligase activities and mediates monoubiquitination of diverse target proteins. Related references: PMID:23455153, PMID:24703950
• Acts as a positive regulator of BMP7 signaling by monoubiquitinating SMAD6, thereby regulating adipogenesis. Related references: PMID:23455153
• Negatively regulates TRAF6-mediated NF-κB activation independently of its E2 activity. Related references: PMID:23381138
• Monoubiquitinates the nuclear localization signal (NLS) of BAP1, causing cytoplasmic retention, and similarly regulates INO80 and CXXC1 localization. Related references: PMID:24703950
• Assists the TRIM27:MAGEL2 E3 ligase complex in mediating Lys-63-linked ubiquitination of WASHC1 to promote endosomal F-actin assembly and retrograde transport. Related references: PMID:23452853
• Monoubiquitinates cytoplasmic CTNNA1 to trigger a binding switch from β-catenin to vinculin, driving cell-to-extracellular matrix adhesion. Related references: PMID:40983751
• Found in both cytoplasm and nucleus; predominantly expressed in skeletal muscle and heart. Phosphorylated and contains ATP-binding and coiled-coil domains.

Experimental Guidance and Technical Tips

• The immunogen covers residues 1073–1292 near the C‑terminus; consider peptide competition to verify band identity in new sample systems.
• In WB, expect a dominant band at approximately 141 kDa. Use KO‑validated lysates as a negative control to confirm specificity.
• For IF/ICC, optimize permeabilization and fixation since UBE2O shuttles between cytoplasm and nucleus.
• For IP, confirm compatibility of the rabbit polyclonal antibody with protein A/G resins and validate elution conditions.
• ELISA may be used for titer determination or sandwich pair screening; pair with a detection antibody recognizing a distinct epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD raw materials, offering this anti‑UBE2O polyclonal antibody for ubiquitination and signaling research. We provide a full portfolio of validated antibodies, bulk reagents, and custom sourcing services to support diagnostic manufacturers from concept to clinical application. Rely on CamelBio for scalable, consistent raw materials and expert technical consultation.