Anti-Ube2N/Ubc13 Rabbit Polyclonal Antibody for WB and ELISA - P61088

Core Product Specifications and Parameters

Biological Background: Ube2N/Ubc13 Function and Localization

• Ube2N (also known as Ubc13, UbcH13) is a ubiquitin-conjugating enzyme E2 that forms a heterodimer with UBE2V1 or UBE2V2 to catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not target proteins for proteasomal degradation but instead serves regulatory roles in signaling, DNA repair, and cell cycle control.
• The enzyme plays a critical role in the error-free DNA damage tolerance pathway by mediating 'Lys-63'-linked polyubiquitination of PCNA in conjunction with E3 ligases HLTF and SHPRH, which is essential for DNA repair and cell survival after genotoxic stress.
• Ube2N is involved in innate immune signaling: together with RNF135 and UBE2V1, it catalyzes viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I to activate interferon beta production (PubMed:28469175). Additionally, the UBE2V1-UBE2N heterodimer promotes TRIM5 capsid-specific restriction activity by generating polyubiquitin chains that activate the TAK1 kinase complex, leading to NF-kB and MAPK-responsive inflammatory gene expression (PubMed:31006531). Related references: PMID:28469175 PMID:31006531
• Subcellularly, Ube2N localizes to both the nucleus and cytoplasm, consistent with its diverse functions in DNA repair (nuclear) and cytoplasmic signaling pathways.
• The protein is subject to post-translational modifications including acetylation and disulfide bond formation, and it functions via isopeptide bond formation during ubiquitin transfer. It belongs to the E2 ubiquitin-conjugating enzyme family and is classified under the Ubl conjugation pathway.
• Ube2N is involved in transcriptional activation of target genes and plays a role in cell cycle progression and differentiation. It also participates in the IL-17A signaling pathway by mediating 'Lys-63'-linked polyubiquitination of TRAF6 together with TRAF3IP2.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody encompasses the full-length human Ube2N protein (aa 1–152). This design may enable recognition of multiple epitopes, potentially providing robust detection even if some epitopes are masked or denatured. However, cross-reactivity with other E2 enzymes sharing conserved regions should be considered; validation in the relevant sample system is recommended.
• The antibody is validated for Western blotting (WB) and ELISA by the supplier. For WB, a band near 17 kDa is expected. It is advisable to confirm specificity using positive and negative control lysates, particularly from human, mouse, or rat cells, given the reported cross-reactivity.
• Given Ube2N's roles in diverse pathways, researchers can explore its dynamic expression or modification under conditions of DNA damage, innate immune stimulation, or cell cycle arrest. Endogenous detection levels may vary; optimization of antibody dilution and exposure times may be necessary.
• For ELISA applications, pairing with a suitable capture antibody or antigen may help establish quantitative assays. Consider evaluating linearity and sensitivity using recombinant Ube2N protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials and technical support, from early-stage development to clinical application. This anti-Ube2N/Ubc13 antibody supports researchers exploring ubiquitination pathways, DNA repair, and immune signaling, and CamelBio can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. With a commitment to bridging innovative research and reliable manufacturing, CamelBio stands as a trusted partner for your IVD raw-material needs.