Anti-Ube2N/Ubc13 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61088

Core Product Specifications and Parameters

Biological Background: Ube2N/Ubc13 Function and Localization

• Ube2N (also known as Ubc13) is an E2 ubiquitin-conjugating enzyme that forms functional heterodimers with the non‑catalytic subunits UBE2V1 or UBE2V2.
• The UBE2V1–UBE2N and UBE2V2–UBE2N complexes specifically catalyze the assembly of non‑canonical Lys‑63‑linked polyubiquitin chains, which do not target substrates for proteasomal degradation.
• It plays an essential role in error‑free DNA damage tolerance by mediating Lys‑63‑linked polyubiquitination of PCNA, in cooperation with the E3 ligases HLTF and SHPRH.
• In antiviral innate immunity, the UBE2N‑UBE2V1 heterodimer, together with the E3 ligase RNF135, catalyzes viral RNA‑dependent Lys‑63‑linked polyubiquitination of RIG‑I, a critical step for downstream interferon‑beta production. Related references: PMID:28469175, PMID:31006531
• The enzyme also functions in NF‑κB and MAPK inflammatory signaling; for instance, the UBE2V1‑UBE2N dimer acts in concert with TRIM5 to generate Lys‑63‑linked chains that activate the TAK1 kinase complex, while together with TRAF3IP2 it mediates TRAF6 polyubiquitination downstream of IL‑17A.
• Ube2N localizes to both the nucleus and the cytoplasm, consistent with its dual roles in nuclear DNA repair and cytoplasmic signal transduction.
• Post‑translational modifications include acetylation and disulfide bond formation; the mature protein has a calculated molecular weight of ~17 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide within the first 100 amino acids of human Ube2N; the immunogen sequence is highly conserved in mouse and rat, supporting the reported cross‑reactivity.
• For Western blotting, a band at approximately 17 kDa is expected; whole‑cell lysates from human, mouse, or rat cell lines are appropriate samples.
• In immunohistochemistry (IHC‑P), antigen retrieval may be required; validate staining in tissues known to express Ube2N, and use appropriate controls.
• For immunofluorescence (IF/ICC), confirm nuclear and cytoplasmic localization; optimization of fixation and permeabilization conditions is recommended.
• For ELISA applications, the antibody can be paired with a suitable detection system; consider using recombinant Ube2N protein or the immunizing peptide as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing partner for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of IVD raw materials, from validated monoclonal and polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. This Ube2N/Ubc13 rabbit monoclonal antibody supports research in the ubiquitin‑proteasome system, DNA repair, and innate immune signaling—areas of high diagnostic interest. Whether you need matched antibody pairs, custom production, or technical consulting to move from concept to clinic, CamelBio is your reliable B2B supply partner.