Anti-UBE2NL Polyclonal Antibody for WB, ELISA - Q5JXB2

Core Product Specifications and Parameters

Biological Background: UBE2NL Function and Localization

• UBE2NL (Putative ubiquitin-conjugating enzyme E2 N-like) is a protein belonging to the ubiquitin-conjugating enzyme (E2) family, predicted to participate in ubiquitin transfer reactions.
• It is also known as epididymis tissue protein Li 174, based on its initial identification in epididymis proteomic studies.
• Tissue specificity: UBE2NL is expressed in the epididymis at the protein level, suggesting a role in reproductive tissue function.
• Keywords associated with UBE2NL include Acetylation, indicating potential post-translational modification, and Reference proteome, marking it as part of the curated human proteome.
• The protein has a calculated molecular weight of approximately 17 kDa, consistent with a small E2 enzyme.
• Although no functional studies are explicitly recorded, the conserved E2 domain implies a role in ubiquitination pathways that may be relevant to epididymal physiology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-100 amino acid region, which may share homology between human and mouse sequences, supporting the observed cross-reactivity.
• For Western blot, detection of a band near 17 kDa is anticipated; consider using positive control lysates from epididymis or other relevant tissues.
• For ELISA, optimize coating conditions and include appropriate negative controls to assess specificity.
• Validate antibody performance in your target species (human/mouse) using recombinant protein or tissue extracts known to express UBE2NL.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For targets like UBE2NL, involved in ubiquitination and reproductive biology, we facilitate procurement of hard-to-find research tools and offer technical consulting to streamline your development pipeline.