Anti-UBE2L6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O14933

Core Product Specifications and Parameters

Biological Background: UBE2L6 Function and Localization

• UBE2L6 (Ubiquitin/ISG15-conjugating enzyme E2 L6), also known as RIG-B and UbcH8, is a member of the ubiquitin-conjugating enzyme E2 family.
• It catalyzes the covalent attachment of ubiquitin or the ubiquitin-like protein ISG15 to target proteins, playing a key role in protein modification and degradation.
• UBE2L6 functions in the E6/E6-AP-induced ubiquitination of the tumor suppressor protein p53/TP53, contributing to its proteasomal degradation.
• It also promotes the ubiquitination and subsequent proteasomal degradation of the receptor tyrosine kinase FLT3, implicating it in signal transduction regulation.
• At the protein level, UBE2L6 is present in natural killer cells, suggesting a role in immune cell function.
• The UBE2L6 gene undergoes alternative splicing, yielding multiple transcript variants that may encode distinct protein isoforms.
• As a transferase enzyme, UBE2L6 is part of the ubiquitin-like (Ubl) conjugation pathway, essential for cellular protein homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within amino acids 100-153 of human UBE2L6. This central region may be conserved; verify sequence homology when using mouse samples.
• For Western blot, the predicted molecular weight is ~18 kDa. Use a high-percentage gel and optimize transfer for small proteins. Alternative splicing or modifications may produce additional bands.
• In IHC-P, optimize antigen retrieval and antibody dilution. Natural killer cell-containing tissues can serve as positive controls.
• For ELISA, consider pairing with a matched detection antibody. Validate in the relevant sample matrix for intended use.
• Ubiquitination or co-immunoprecipitation assays could be explored to study UBE2L6 substrate interactions, though not validated for this product.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering this anti-UBE2L6 polyclonal antibody alongside a full portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services to support your ubiquitination and cancer research assay development from concept to clinic.