Anti-UBE2L6 Polyclonal Antibody for WB, IF/ICC, ELISA - O14933

Core Product Specifications and Parameters

Biological Background: UBE2L6 Function and Localization

• UBE2L6 is a ubiquitin/ISG15-conjugating enzyme E2 L6, also known as RIG-B or UbcH8, that plays a dual role in ubiquitin and ISG15 modification pathways.
• It catalyzes the covalent attachment of ubiquitin or ISG15 to substrate proteins, facilitating protein degradation or signaling.
• Functions in the E6/E6-AP-induced ubiquitination of p53/TP53, contributing to the regulation of tumor suppressor stability.
• Promotes ubiquitination and subsequent proteasomal degradation of FLT3, a receptor tyrosine kinase important in hematopoiesis.
• UBE2L6 is present in natural killer cells at the protein level, suggesting a role in immune function.
• Alternative splicing of UBE2L6 produces multiple isoforms, and its structure has been solved by X-ray crystallography (3D-structure keyword).
• Classified as a transferase enzyme in the ubiquitin-like conjugation pathway, essential for protein homeostasis and signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from an internal region (aa 100-153); thus, the antibody may recognize multiple isoforms containing this sequence.
• For Western blot, expect a specific band at approximately 18 kDa corresponding to UBE2L6. Validate with positive controls from relevant tissues or cell lines.
• In immunofluorescence/ICC, test in natural killer cells or cell lines expressing UBE2L6 to confirm localization.
• For ELISA, consider using peptide blocking to assess antibody specificity.
• Optimal working dilutions and conditions should be empirically determined for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical support. This anti-UBE2L6 polyclonal antibody supports studies on ubiquitin and ISG15 conjugation pathways, ideal for cancer and immunology research. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for assay development, as well as sourcing of rare target raw materials to streamline your project from concept to clinic.