Anti-Ube2L3 / UBCH7 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P68036

Core Product Specifications and Parameters

Biological Background: Ube2L3 / UBCH7 Function and Localization

• UBE2L3 is a ubiquitin-conjugating enzyme E2 that specifically partners with HECT-type and RBR family E3 ubiquitin ligases, including PRKN and RNF31. It lacks intrinsic reactivity with lysine for most RING-type E3s.
• It accepts ubiquitin from the E1 enzyme and catalyzes covalent attachment to target proteins, mediating ubiquitination via complexes such as CUL9-RBX1 (PubMed:38605244). Related references: PMID:38605244
• UBE2L3 catalyzes 'Lys-11'-linked polyubiquitination in vitro and plays a key role in the selective degradation of short-lived and abnormal proteins.
• The enzyme is down-regulated during S-phase and is involved in cell cycle progression, as well as in the regulation of nuclear hormone receptor transcriptional activity.
• It may also play a role in myelopoiesis.
• The protein is localized to both the nucleus and cytoplasm, and is ubiquitously expressed with highest levels in testis.
• UBE2L3 is subject to acetylation and alternative splicing, and is identified as a reference proteome component involved in transcription regulation and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide spanning amino acids 55-154 of human UBE2L3. For applications, consider the reported molecular weight of approximately 18 kDa for target detection in Western blotting.
• Given its reactivity with human, mouse, and rat samples, this antibody may be suitable for cross-species studies; however, validation in each species is recommended.
• For immunohistochemistry (IHC) and immunofluorescence (IF/ICC), the nuclear and cytoplasmic localization of UBE2L3 suggests that permeabilization steps should be optimized to ensure detection in both compartments.
• For ELISA and other sensitive assays, antibody titration is advised to maximize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This UBE2L3/UBCH7 rabbit monoclonal antibody is part of our extensive portfolio of ubiquitin pathway reagents, available alongside validated antibody pairs and optimized antibodies for various applications, supporting your assay development from concept to clinic.