Anti-UBE2J2 Polyclonal Antibody for WB, ELISA - Q8N2K1

Core Product Specifications and Parameters

Biological Background: UBE2J2 Function and Localization

• UBE2J2, also known as NCUBE-2 or UBC6, is an E2 ubiquitin-conjugating enzyme that catalyzes the covalent attachment of ubiquitin to target proteins, playing a key role in protein post-translational modification.
• It functions in the endoplasmic reticulum-associated degradation (ERAD) pathway, likely facilitating the selective degradation of misfolded membrane proteins from the ER.
• In cooperation with the GATOR2 complex, UBE2J2 specifically catalyzes 'Lys-6'-linked ubiquitination of NPRL2, a component of the GATOR1 complex, linking ubiquitin signaling to mTORC1 regulation.
  Related references:
  PMID:36528027
• The enzyme is anchored to the endoplasmic reticulum membrane via a transmembrane helix, and its subcellular localization is critical for its function in ER protein quality control.
• Alternative splicing of UBE2J2 produces multiple isoforms, and the protein contains structural features important for its interaction with ubiquitin and E1/E3 enzymes.
• UBE2J2 is associated with the unfolded protein response (UPR) pathway, indicating a role in cellular stress responses.
• The predicted molecular weight is approximately 28.9 kDa, with an observed band around 29 kDa in SDS-PAGE, consistent with immunoblotting data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human UBE2J2 (residues 1-230), suggesting the antibody may recognize both endogenous and recombinant protein forms.
• For Western blot detection, expect a specific band near 29 kDa. To confirm specificity, consider using lysates from human, mouse, or rat cell lines, as well as siRNA knockdown or overexpression controls.
• For ELISA assays, use the recombinant protein as a standard and validate the linear range in the relevant sample matrix.
• Optimal working dilutions should be determined empirically; start with recommended ranges for polyclonal antibodies in similar applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For targets like UBE2J2, which are involved in protein degradation and stress response pathways, we offer bulk antibodies and custom sourcing to support assay development and validation. Rely on CamelBio to streamline your supply chain from early research to clinical application.