Anti-UBE2H Polyclonal Antibody for WB and ELISA - P62256

Core Product Specifications and Parameters

Biological Background: Ubiquitin-conjugating enzyme E2 H Function and Localization

• UBE2H (Ubiquitin-conjugating enzyme E2 H) is an E2 enzyme that accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to substrate proteins, a central step in ubiquitin-mediated signaling (PubMed:17588522, PubMed:20061386, PubMed:8132613). Related references: PMID:17588522, PMID:20061386, PMID:8132613

• It transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex, facilitating targeted protein degradation or functional modulation (PubMed:29911972). Related references: PMID:29911972

• In vitro, UBE2H catalyzes the formation of 'Lys-11'-linked and 'Lys-48'-linked polyubiquitin chains, which are associated with proteasomal degradation and non-degradative signaling (PubMed:20061386). Related references: PMID:20061386

• It can directly ubiquitinate histone H2A in vitro, suggesting a potential role in chromatin modification and epigenetic regulation (PubMed:8132613). Related references: PMID:8132613

• According to UniProt keywords, UBE2H is subject to acetylation and alternative splicing, which may produce multiple isoforms with distinct regulatory functions.

• The mature UBE2H protein has a calculated molecular mass of approximately 20.7 kDa (UniProt), consistent with the observed 21 kDa band in immunoblotting.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human UBE2H (amino acids 1–183). This design suggests reactivity with native UBE2H across species, supported by the reported cross-reactivity with human, mouse, and rat.
• For Western blotting, a predominant band around 21 kDa is expected. Consider loading controls and perform preliminary validation in your specific sample matrix to confirm specificity.
• In ELISA applications, optimization of coating antigen concentration, blocking conditions, and secondary antibody dilution may be required to achieve optimal signal-to-noise ratios.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays. We recommend aliquoting and storing at recommended temperatures to maintain stability.
• Cross-reactivity with mouse and rat UBE2H has been observed; for other species, consult sequence homology and conduct a pilot test if cross-reactivity is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-UBE2H polyclonal antibody supports research in ubiquitin-proteasome pathways and related diagnostic development. In addition to primary antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and sourcing of rare-target raw materials to accelerate your assay development and production.