Anti-UBE2G2 Polyclonal Antibody for WB, IF/ICC, ELISA - P60604

Core Product Specifications and Parameters

Biological Background: UBE2G2 Function and Localization

• UBE2G2 (Ubiquitin-conjugating enzyme E2 G2), also known as UBC7, is an E2 enzyme that accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to substrate proteins.
• It catalyzes 'Lys-48'-linked polyubiquitination, a canonical signal for proteasomal degradation. Related references: PMID:20061386
• UBE2G2 plays a critical role in endoplasmic reticulum-associated degradation (ERAD), facilitating the clearance of misfolded proteins from the ER. Related references: PMID:22607976
• The enzyme is required for sterol-induced ubiquitination and subsequent proteasomal degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the rate-limiting enzyme in cholesterol biosynthesis. Related references: PMID:23223569
• Subcellularly, UBE2G2 localizes to the endoplasmic reticulum and lipid droplets, reflecting its dual roles in protein quality control and lipid metabolism.
• This protein belongs to the ubiquitin-conjugating enzyme family and is subject to alternative splicing and acetylation, with a predicted molecular weight of approximately 19 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-165) of human UBE2G2; this region is highly conserved across human, mouse, and rat, supporting its reported cross-reactivity.
• For Western blotting, a band around 19 kDa is expected; however, post-translational modifications or isoform variations may result in additional bands or shifts—optimize antibody dilution and detection conditions for your specific sample.
• In immunofluorescence/immunocytochemistry, consider appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., Triton X-100) to detect ER and lipid droplet localization; validate staining in UBE2G2-expressing cell lines.
• This polyclonal antibody, generated in rabbit, may offer higher sensitivity for detecting low-abundance endogenous UBE2G2, but careful titration is recommended to minimize non-specific binding.
• When using for ELISA, ensure the recombinant protein or peptide corresponds to the immunogen region for optimal capture/detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-UBE2G2, or ancillary reagents for ubiquitin pathway and ERAD research, we support your project from concept to clinic. Our global sourcing capabilities cover rare targets and custom antibody development to accelerate your diagnostic assay development.