Anti-UBE2E2 Polyclonal Antibody for WB, ELISA - Q96LR5

Core Product Specifications and Parameters

Biological Background: UBE2E2 Function and Localization

• UBE2E2, also known as UbcH8, is a member of the ubiquitin-conjugating enzyme E2 family that plays a critical role in the ubiquitin-proteasome system.
• It accepts ubiquitin from the E1 activating enzyme and catalyzes its covalent attachment to target proteins, forming polyubiquitin chains via Lys-11, Lys-48, and Lys-63 linkages in vitro.
• UBE2E2 mediates the ISGylation of influenza A virus NS1 protein, suggesting a role in antiviral innate immune responses.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity or interactions.
• UBE2E2 is classified under the transferase family, specifically involved in the ubiquitin-conjugating pathway, a key process for protein degradation, signaling, and cellular homeostasis.
• Molecular weight is approximately 22 kDa, consistent with the recombinant protein used as immunogen.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (aa 1-201), so the antibody is expected to detect both native and denatured UBE2E2; consider testing in your specific sample type.
• Validated for Western blot (WB) and ELISA; users should perform initial titration experiments to determine optimal antibody dilution for their application.
• Cross-reacts with mouse and rat UBE2E2, making it suitable for rodent model studies, but confirm reactivity in your samples before large-scale experiments.
• As a polyclonal antibody, it may exhibit lot-to-lot variability; include positive and negative controls in each assay to ensure consistent performance.
• For WB, the predicted molecular weight is ~22 kDa; use appropriate molecular weight markers and consider blocking conditions to minimize nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This UBE2E2 polyclonal antibody supports studies on ubiquitin-proteasome pathways, viral host interactions, and protein degradation research. As a one-stop sourcing partner, CamelBio ensures timely availability of validated reagents like this antibody, along with bulk formats and technical support, to accelerate your diagnostic and research projects from concept to clinic.