Anti-UBE2D4 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y2X8

Core Product Specifications and Parameters

Biological Background: UBE2D4 Function and Localization

• UBE2D4, also known as HBUCE1, ubiquitin carrier protein D4, or ubiquitin-protein ligase D4, is an E2 ubiquitin-conjugating enzyme encoded by the UBE2D4 gene (Gene ID: 51619) in humans.
• The core function of UBE2D4 is to accept ubiquitin from the E1 activating enzyme and catalyze its covalent attachment to lysine residues on target proteins, a key step in the ubiquitination cascade.
• In vitro, UBE2D4 can promote polyubiquitination using all seven internal lysine residues of ubiquitin, with a preference for Lys-11- and Lys-48-linked chain formation, which are classically associated with proteasomal degradation and cellular signaling.
• The protein has a calculated molecular weight of approximately 16.6 kDa (UniProt), while the full-length recombinant immunogen (amino acids 1-147) yields an observed band around 17 kDa in Western blotting.
• According to UniProt, UBE2D4 has been identified in proteomic studies, confirming its expression in human cells and supporting its role as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full coding sequence (aa 1-147) of human UBE2D4, so the antibody is expected to recognize the intact, native protein; for validation, include a positive control lysate known to express UBE2D4.
• For Western blot (WB), the predicted band size is approximately 17 kDa; use a gel percentage appropriate for low-molecular-weight proteins and confirm with molecular weight markers. The antibody detects human, mouse, and rat UBE2D4, enabling cross-species studies.
• In ELISA, this polyclonal antibody can serve as a capture or detection reagent; optimal dilution should be determined empirically by titration using recombinant UBE2D4 or cell lysates.
• As a polyclonal antibody, lot-to-lot variation is possible; always perform initial titration experiments and include appropriate controls. Store at –20°C upon receipt (shipped on ice bags) and centrifuge briefly before first use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw materials, offering validated polyclonal antibodies like this anti-UBE2D4 product, along with matched antibody pairs, optimized monoclonals, and bulk ancillary reagents for ubiquitination research. With extensive supply chain capabilities, we support diagnostic developers and laboratories in efficiently sourcing rare-target materials and scaling from early-stage R&D to clinical applications, ensuring reliable and consistent raw material supply.