Anti-UBE2D2 Polyclonal Antibody for WB - P62837

Core Product Specifications and Parameters

Biological Background: UBE2D2 Function and Localization

• Ubiquitin-conjugating enzyme E2 D2 (UBE2D2), also known as UBC4, UBCH5B, and PUBC1, is a member of the ubiquitin-conjugating enzyme family.
• Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins, a key step in the ubiquitination cascade. Related references: PMID:10329681, PMID:18042044, PMID:18703417
• Catalyzes 'Lys-48'-linked polyubiquitination, mediating the selective degradation of short-lived and abnormal proteins via the proteasome. Related references: PMID:10329681, PMID:18042044, PMID:18359941
• Functions in the E6/E6-AP-induced ubiquitination of p53/TP53, promoting its degradation. Related references: PMID:15280377
• Mediates ubiquitination of various substrates, including PEX5, SQSTM1, STUB1, and TRAF6, regulating diverse cellular processes. Related references: PMID:18359941, PMID:28322253
• Involved in signal-induced conjugation and degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination, MDM2-dependent degradation of p53/TP53, and RIGI-mediated MAVS activation during viral infection. Related references: PMID:18703417, PMID:20403326
• Essential for viral activation of IRF3, playing a critical role in innate immune response. Related references: PMID:19854139
• Alternative splicing produces multiple isoforms; classified as a transferase in the ubiquitin-conjugation pathway (UniProt).

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human UBE2D2 protein (aa 1-147), which is highly conserved; verify sequence alignment for your species of interest to ensure cross-reactivity.
• For WB, a band near 17 kDa is expected; consider using a 12-15% SDS-PAGE gel and optimized transfer conditions for low molecular weight proteins.
• Reported cross-reactivity with human, mouse, and rat; confirm expression in your specific cell lines or tissue samples before large-scale experiments.
• As a polyclonal antibody, batch-to-batch variability may occur; include appropriate positive and negative controls in each assay.
• For ELISA, titrate the antibody against your coating antigen to determine the optimal dilution for maximum signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting. For ubiquitin pathway targets like UBE2D2, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions. Partner with us to accelerate your assay development from concept to clinic.