Anti-UBE2D1/SFT Rabbit Monoclonal Antibody for IHC-P and ELISA - P51668

Core Product Specifications and Parameters

Biological Background: UBE2D1 (SFT/UbcH5) Function and Localization

• The UBE2D1 gene encodes Ubiquitin-conjugating enzyme E2 D1 (also known as SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1), a member of the E2 ubiquitin-conjugating enzyme family that transfers ubiquitin from the E1 complex to substrate proteins, mediating selective degradation of short-lived and abnormal proteins. Related references: PMID:22496338

• It catalyzes 'Lys-48'-linked polyubiquitination in vitro, a key signal for proteasomal degradation, and functions in E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Related references: PMID:20061386, PMID:18042044, PMID:18359941

• Ubiquitinates STUB1-associated HSP90AB1 in vitro and lacks inherent specificity for any particular lysine residue of ubiquitin. Related references: PMID:18042044

• Essential for viral activation of IRF3 and mediates polyubiquitination of CYP3A4. Related references: PMID:19854139, PMID:19103148

• Localizes to the cytoplasm and is ubiquitously expressed, with up-regulation in livers of iron-overloaded patients with hereditary hemochromatosis, consistent with its alias Stimulator of Fe transport (SFT).

• Plays a role in the Ubl conjugation pathway as a transferase; its 3D-structure has been determined, revealing key catalytic features.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 50-147) corresponds to the conserved core domain of human UBE2D1, suggesting that the antibody may recognize both denatured and native forms; validation in the relevant sample system is recommended.
• For IHC-P, consider heat-induced epitope retrieval and antibody optimization due to potential antigen masking; tissue-specific expression patterns should be confirmed with appropriate controls.
• In ELISA applications, the monoclonal nature may provide high specificity; it can be evaluated as a capture or detection agent following standard optimization protocols.
• Cross-reactivity with mouse and rat UBE2D1 orthologs (predicted based on sequence homology) should be experimentally verified if used in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Discover how our UBE2D1/SFT rabbit monoclonal antibody and related ubiquitin-pathway research reagents can accelerate your diagnostic development from concept to clinic.