Anti-UBE2C Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O00762

Core Product Specifications and Parameters

Biological Background: UBE2C Function and Localization

• UBE2C (UBCH10) is a member of the ubiquitin-conjugating enzyme (E2) family, responsible for accepting ubiquitin from the E1 enzyme and catalyzing its transfer to substrates.
• The protein is an essential cofactor of the anaphase promoting complex/cyclosome (APC/C), a critical E3 ubiquitin ligase that regulates mitotic progression by targeting key substrates for proteasomal degradation.
• UBE2C primarily catalyzes 'Lys-11'-linked polyubiquitin chains, a non-canonical linkage that is crucial for APC/C substrate recognition and subsequent degradation.
• In addition to Lys-11 chains, UBE2C can also mediate 'Lys-48'-linked polyubiquitination in vitro, highlighting its versatility in ubiquitin chain formation.
• Alternative splicing of the UBE2C gene may generate multiple transcript variants, potentially yielding isoforms with distinct regulatory properties.
• Post-translational modifications, including phosphorylation and acetylation, are indicated by UniProt keywords, suggesting dynamic regulation of UBE2C activity during the cell cycle.
• The protein is highly conserved across species, allowing cross-reactivity with mouse and rat orthologs, as confirmed by the antibody validation.

Experimental Guidance and Technical Tips

• The full-length immunogen (aa 1-179) may enable recognition of all UBE2C isoforms; therefore, bands of slightly different molecular weights might be observed in some tissues or cell states.
• For Western blotting, use mitotic-arrested cell lysates (e.g., nocodazole treatment) as a positive control, as UBE2C levels peak during mitosis. A band near 20 kDa is expected.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider heat-induced antigen retrieval with citrate buffer (pH 6.0) and validate in known positive tissues, such as highly proliferative tumors or testis.
• In immunoprecipitation (IP), the polyclonal antibody can enrich UBE2C and its interacting partners; pre-clearing with control IgG is recommended to reduce non-specific binding.
• For ELISA, optimize coating antigen concentration and antibody dilution using recombinant UBE2C protein as a standard; note that polyclonal antibodies may exhibit slight lot-to-lot variability.
• Always include relevant controls (e.g., UBE2C knockout lysates) to confirm specificity, particularly when working with samples where UBE2C expression is low or regulated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with seamless access to premium IVD raw materials, from monoclonal and polyclonal antibodies to ancillary reagents and custom sourcing. This anti-UBE2C product exemplifies our focus on cell cycle and ubiquitin‑proteasome pathway targets, supporting assay development in oncology and cell biology. Whether you need validated antibody pairs for sandwich ELISA, bulk production-grade conjugates, or assistance with rare target procurement, our integrated supply chain accelerates your project from concept to clinic.