Anti-UBE2C Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O00762

Core Product Specifications and Parameters

Biological Background: UBE2C Function and Localization

• UBE2C (Ubiquitin-conjugating enzyme E2 C), also known as UbcH10, is an E2 ubiquitin-conjugating enzyme that plays a critical role in the ubiquitin-proteasome system.
• It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to target proteins, primarily forming Lys-11- and Lys-48-linked polyubiquitin chains in vitro.
• UBE2C is an essential cofactor for the anaphase promoting complex/cyclosome (APC/C), a key E3 ligase that regulates mitotic progression by targeting cell cycle proteins for degradation.
• By initiating Lys-11-linked ubiquitin chains on APC/C substrates, UBE2C promotes their proteasomal degradation, facilitating mitotic exit.
• The protein is regulated by alternative splicing and post-translational modifications, including acetylation and phosphorylation, which may modulate its activity during cell division.
• Keywords associated with UBE2C include 3D-structure, acetylation, alternative splicing, cell cycle, cell division, mitosis, phosphoprotein, transferase, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen is described as a recombinant protein corresponding to amino acids 1–179 of human TLR4. Users should verify the antibody's specificity for UBE2C using appropriate positive and negative controls, as the immunogen does not correspond to the UBE2C protein.
• For Western blot (WB), a band at approximately 20 kDa is expected. Optimization of antibody dilution and protein loading is recommended.
• In immunohistochemistry (IHC-P), tissue-specific expression should be confirmed using known positive control tissues (e.g., proliferating cancer cell lines or tissues with active cell division).
• For ELISA, pairing with a suitable capture/detection reagent may enhance assay performance; consider validating antibody affinity and binding in the relevant buffer system.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio streamlines procurement for diagnostic manufacturers and research laboratories. This UBE2C antibody supports cell cycle and oncology research, and CamelBio can further provide validated antibody pairs, recombinant proteins, and ancillary reagents to advance your assay development. From concept to clinic, our one-stop platform offers expert technical consultation and tailored sourcing solutions for rare targets and bulk requirements.