Anti-UBE2C Polyclonal Antibody for WB and ELISA - O00762

Core Product Specifications and Parameters

Biological Background: UBE2C Function and Localization

• UBE2C (also known as UBCH10) is a ubiquitin-conjugating enzyme E2 that accepts ubiquitin from E1 and catalyzes its covalent attachment to substrate proteins.
• It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis.
• UBE2C initiates 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to their degradation by the proteasome and promoting mitotic exit.
• The enzyme demonstrates E3-independent activity in vitro, catalyzing both 'Lys-11'- and 'Lys-48'-linked polyubiquitination.
• Keywords associated with UBE2C include acetylation, phosphoprotein, alternative splicing, and proteomics identification, indicating post-translational modifications and regulatory mechanisms.
• The protein has a calculated molecular weight of approximately 19.7 kDa and is conserved in the ubiquitin-conjugating enzyme family.

Experimental Guidance and Technical Tips

• For Western blot, consider standard SDS-PAGE and transfer protocols. Validate detection in samples with known UBE2C expression, as its levels fluctuate during the cell cycle.
• For ELISA, this polyclonal antibody can be used to capture or detect recombinant or endogenous UBE2C. Optimize coating and detection antibody concentrations through titration.
• Given the immunogen is a recombinant protein spanning amino acids 1-179, the antibody may recognize both native and denatured epitopes. Include appropriate positive and negative controls in your assays.
• Since UBE2C is a low-molecular-weight protein (~20 kDa), ensure the use of high-resolution gels and appropriate molecular weight markers for accurate band identification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing IVD raw materials, including validated antibodies like this anti-UBE2C product, to support diagnostic assay development in cancer and cell cycle research. We offer a comprehensive portfolio of antibody pairs, recombinant proteins, and ancillary reagents, and can assist with sourcing rare-target raw materials. Partner with CamelBio to streamline your procurement needs from early-stage development to final product realization.