Anti-UBE2B Polyclonal Antibody for WB, ELISA - P63146

Core Product Specifications and Parameters

Biological Background: UBE2B Function and Localization

• UBE2B is an E2 ubiquitin-conjugating enzyme that accepts ubiquitin from E1 and transfers it to E3 ligases, catalyzing Lys-11, Lys-48, and Lys-63 polyubiquitination in vitro. Related references: PMID:20061386
• Together with the E3 enzyme BRE1 (RNF20/RNF40), UBE2B catalyzes monoubiquitination of histone H2B at Lys-120 (H2BK120ub1), a mark essential for epigenetic transcriptional activation, RNA polymerase II elongation, telomeric silencing, and a prerequisite for H3K4 and H3K79 methylation. Related references: PMID:16337599
• In DNA repair, UBE2B partners with RAD18 to monoubiquitinate PCNA at Lys-164, facilitating DNA damage tolerance. Related references: PMID:17108083, PMID:17130289
• UBE2B is involved in the N-end rule pathway through association with UBR4 ligase, mediating selective protein degradation. Related references: PMID:38182926
• Early studies indicate UBE2B plays a role in general DNA repair processes. Related references: PMID:8062904
• Subcellularly, UBE2B localizes to the cell membrane and nucleus.
• Keywords associated with UBE2B include 3D-structure, DNA damage, DNA repair, membrane, nucleus, transferase, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human UBE2B (amino acids 1–152), suggesting the polyclonal antibody may recognize multiple epitopes across the native protein in denaturing and native conditions.
• For Western blot, consider using a positive control lysate from HeLa or HEK293 cells, where UBE2B is expressed at expected molecular weight of 17 kDa.
• Validate antibody specificity by siRNA knockdown or by comparing signal intensity in relevant tissue or cell models with known UBE2B expression levels.
• For ELISA, optimal coating antigen concentration and detection system should be empirically determined; recombinant full-length UBE2B or synthetic peptide can be used for assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. With this high-quality anti-UBE2B polyclonal antibody, researchers can confidently explore ubiquitin pathway functions, epigenetic regulation, and DNA repair mechanisms. Contact us for custom antibody conjugation, recombinant protein expression, or rare target sourcing to accelerate your project timelines.