Anti-UBE2A Polyclonal Antibody for WB, IHC-P, ELISA - P49459

Core Product Specifications and Parameters

Biological Background: UBE2A Function and Localization

• UBE2A functions as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 enzyme and transferring it to an E3 ubiquitin-protein ligase, playing a central role in the ubiquitination cascade. Related references: PMID:16337599, PMID:20061386, PMID:23685073
• In vitro, UBE2A catalyzes both 'Lys-11'- and 'Lys-48'-linked polyubiquitination, indicating versatility in chain linkage specificity. Related references: PMID:20061386
• Together with the E3 ligase BRE1 (RNF20/RNF40), UBE2A monoubiquitinates histone H2B at Lys-120 (H2BK120ub1), an epigenetic mark critical for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and prerequisite for H3K4 and H3K79 methylation. Related references: PMID:16337599
• UBE2A participates in mitophagy by acting as an E2 enzyme for the E3 ligase PRKN, implicating it in mitochondrial quality control. Related references: PMID:23685073
• In complex with the E3 enzyme UBR4, UBE2A is involved in the N-end rule pathway, directing degradation of proteins bearing specific N-terminal residues. Related references: PMID:38182926
• Through association with the SIFI complex, UBE2A inhibits the mitochondrial stress response, highlighting a regulatory role in cellular proteostasis. Related references: PMID:38297121
• Subcellularly, UBE2A localizes to late endosomes and lysosomes, consistent with its functions in protein trafficking and turnover.
• UBE2A is subject to alternative splicing and phosphorylation, and is classified as a chromatin regulator linked to DNA damage response and intellectual disability (MRXSN).

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human UBE2A protein (residues 1–152), suggesting the polyclonal antibody may recognize multiple epitopes and could detect endogenous UBE2A across various species, including the confirmed human, mouse, and rat cross-reactivities.
• For Western blot (WB), consider the predicted molecular weight of 17kDa; optimize lysis conditions and loading controls to validate band specificity, especially given the small protein size.
• In immunohistochemistry (IHC-P), antigen retrieval and detection conditions should be empirically determined; include appropriate tissue controls and blocking steps to minimize background.
• For ELISA, the polyclonal nature may offer high sensitivity; titration and checkerboard experiments are recommended to establish optimal coating and detection parameters.
• Because UBE2A participates in multiple cellular processes (ubiquitination, chromatin regulation, mitophagy), the antibody can be used to probe these pathways, but results should be corroborated with appropriate functional assays or knockdown/overexpression experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. This anti-UBE2A polyclonal antibody supports investigations into ubiquitin-mediated signaling, epigenetic regulation, and mitochondrial biology. Through a robust global supply network, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, accelerating your assay development and research goals.