Anti-Ube1L / UBA7 Monoclonal Antibody for WB, ELISA - P41226

Core Product Specifications and Parameters

Biological Background: Ube1L / UBA7 Function and Localization

• Ube1L / UBA7, also known as ubiquitin-like modifier-activating enzyme 7, D8, UBE1L, or UBA1B, is an E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein ISG15 to target proteins, a process called ISGylation.
  Related references: PMID:27545325
• It plays a critical role in antiviral immunity by restricting replication of various viruses including rabies virus, influenza virus, sindbis virus, rotavirus, and human cytomegalovirus through ISGylation of viral and host proteins.
  Related references: PMID:16254333, PMID:19073728, PMID:29056542
• ISG15 modification of influenza A protein NS1 disrupts its association with importin-alpha, inhibiting NS1 nuclear import and thus impairing viral replication.
  Related references: PMID:20133869
• ISGylation of human cytomegalovirus protein UL26 regulates its stability and inhibits its ability to suppress NF-kappa-B signaling.
  Related references: PMID:27564865
• Subcellularly, Ube1L / UBA7 localizes to both the cytoplasm and nucleus, consistent with its role in modifying targets in multiple compartments.
• It is expressed in a variety of normal and tumor cell types, but expression is notably reduced in lung cancer cell lines, suggesting a potential tumor-suppressive role.
• The protein contains ATP-binding and nucleotide-binding domains, and is subjected to phosphorylation, as indicated by proteomics identification.

Experimental Guidance and Technical Tips

• The antibody was generated using an N-terminal immunogen (amino acids 1-100). Researchers should verify that the epitope is not masked or altered in their specific experimental conditions.
• For Western blot detection, Ube1L / UBA7 has a molecular weight of approximately 112 kDa. Use a suitable gel percentage (e.g., 8-10% SDS-PAGE) and ensure adequate transfer conditions for high molecular weight proteins.
• Expression of Ube1L / UBA7 is inducible by type I interferons; consider treating cells with interferon-alpha or interferon-beta to augment protein levels for detection.
• The antibody is reactive with human, mouse, and rat samples based on supplier cross-reactivity data. Validate performance in the relevant species and tissue/cell types.
• For ELISA applications, optimize coating conditions and antibody dilutions empirically using the immunogen or recombinant Ube1L / UBA7 protein as a standard.
• Since Ube1L / UBA7 functions in ISGylation, co-detection with ISG15 or ISGylated substrates may be relevant; use appropriate antibodies and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, biotech companies, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this Ube1L / UBA7 antibody for immunology and virology studies. Leveraging our one-stop sourcing model, we offer optimized antibody pairs, bulk ancillary reagents, and custom procurement for rare targets, supporting your project from concept to clinical application.