Anti-UBAC2 Rabbit Polyclonal Antibody for WB, ELISA - Q8NBM4

Core Product Specifications and Parameters

Biological Background: UBAC2 Function and Localization

• UBAC2 (Ubiquitin-associated domain-containing protein 2), also known as PHGDHL1, is an ER-phagy receptor that mediates selective autophagy of endoplasmic reticulum fragments, essential for maintaining ER homeostasis and limiting ER stress-induced inflammatory responses. Related references: PMID:39284914

• Its function is phosphorylation-dependent, promoting dimerization and enhancing interaction with GABARAP family proteins to facilitate recruitment of ER fragments to autophagosomes. Related references: PMID:39284914

• UBAC2 restricts trafficking of FAF2 from the endoplasmic reticulum to lipid droplets, influencing lipid droplet dynamics. Related references: PMID:23297223

• In complex with LMBR1L and the E3 ubiquitin ligase AMFR, UBAC2 negatively regulates the canonical Wnt signaling pathway in lymphocytes by promoting ubiquitin-mediated degradation of CTNNB1 (β-catenin) and Wnt receptors FZD6 and LRP6. Related references: PMID:31073040

• UBAC2 is a single-pass type I transmembrane protein localized predominantly to the endoplasmic reticulum membrane.

• Post-translational modifications include phosphorylation and N-linked glycosylation, consistent with roles in signaling and membrane dynamics.

• Alternative splicing generates multiple transcript variants, potentially expanding its functional repertoire.

• Keywords associated with UBAC2 include Autophagy, Wnt signaling pathway, Endoplasmic reticulum, Membrane, Glycoprotein, Phosphoprotein, and Alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 220–310, so the antibody may detect full-length UBAC2 and certain splice variants if the epitope is conserved; validate in your target isoform.
• For Western blot, the predicted molecular weight is approximately 39 kDa; use appropriate membrane protein extraction protocols (e.g., RIPA buffer) to ensure efficient solubilization.
• Although cross-reactivity with mouse is indicated, test the antibody in your specific model system, as polyclonal antibodies may exhibit lot-to-lot variation.
• For ELISA, consider using the recombinant immunogen as a positive control to establish optimal coating concentrations and detection conditions.
• UBAC2 is an ER-resident protein; include appropriate subcellular fractionation controls if assessing localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research labs, providing one-stop access to IVD raw materials from concept to clinic. Our UBAC2 polyclonal antibody supports investigations into ER-phagy, Wnt signaling, and immune regulation, and we can supply validated antibody pairs, monoclonal options, and bulk ancillary reagents to accelerate your assay development. For rare target raw materials or customized formulations, contact our team to explore seamless procurement solutions.