Anti-UBA6 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - A0AVT1

Core Product Specifications and Parameters

Biological Background: UBA6 Function and Localization

• UBA6, also known as E1-L2, MOP-4, or UBE1L2, is a ubiquitin-like modifier-activating enzyme that operates as an E1 ligase in the ubiquitin-proteasome system.
• It activates ubiquitin by adenylating its C-terminal glycine with ATP and subsequently forms a ubiquitin-E1 thioester, a critical initial step in ubiquitin conjugation. Related references: PMID:35970836 PMID:35986001
• In addition to ubiquitin, UBA6 can also activate the ubiquitin-like modifier FAT10 via adenylation of its C-terminal glycine, linking it to the UBA6 active site cysteine. Related references: PMID:17889673 PMID:35970836 PMID:35986001
• Unlike UBE1, UBA6 exhibits distinct specificity for downstream E2 enzymes; it does not charge cell cycle-associated E2s such as CDC34, indicating a specialized role in ubiquitin pathway regulation.
• UBA6 is essential for embryonic development, and its isoform 2 is predominantly expressed in testis, where it may influence spermatogenesis and male fertility.
• The protein is widely expressed across tissues and contains key functional motifs including an ATP-binding domain, metal-binding sites, and sites for acetylation and phosphorylation.
• Structurally, UBA6 has been characterized by 3D-structure determination, and it participates in the ubiquitin conjugation pathway as part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-260) of human UBA6, which may be critical for enzyme activity; consider this when selecting detection applications.
• For Western blot, the predicted molecular weight is 118 kDa; validate detection in lysates from human or mouse cell lines, and consider using a high-molecular-weight marker.
• For immunoprecipitation, confirm complex formation under native conditions in relevant biological samples.
• In immunofluorescence/ICC experiments, optimize permeabilization and fixation steps to detect endogenous UBA6, as localization may be influenced by cellular context.
• Although cross-reactivity is confirmed for human and mouse, it is advisable to verify reactivity in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop procurement partner for IVD manufacturers and researchers, offering a comprehensive portfolio of raw materials including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies focused on ubiquitin-activating enzymes like UBA6—relevant to protein degradation, cancer biology, and fertility research—CamelBio can facilitate sourcing of rare and specialized reagents to accelerate your diagnostic development.