Anti-UBA52 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62987

Core Product Specifications and Parameters

Biological Background: UBA52 (Ubiquitin-Ribosomal Protein eL40 Fusion Protein) Function and Localization

• UBA52 encodes a ubiquitin-ribosomal protein eL40 fusion protein, also known as CEP52, RPL40, or HUBCEP52. The precursor is processed to release free ubiquitin and ribosomal protein L40.
• The ubiquitin moiety can form isopeptide linkages with target proteins as monoubiquitin or polyubiquitin chains linked through Lys-6, -11, -29, -33, -48, -63, or linear Met-1, directing diverse processes including proteasomal degradation, DNA repair, endocytosis, and inflammatory signaling.
• As the ribosomal protein L40 component of the 60S subunit, UBA52 facilitates cap-dependent translation of a subset of cellular and viral mRNAs, such as vesicular stomatitis virus transcripts. Related references: PMID:23169626 | PMID:23636399 | PMID:32669547
• UBA52 localizes to both the cytoplasm and nucleus, supporting its dual role in ubiquitin-dependent signaling and ribosomal protein synthesis.
• The protein undergoes multiple post-translational modifications, including phosphorylation, methylation, and ADP-ribosylation, and contains zinc-binding sites critical for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–100, which includes the conserved ubiquitin domain. Consequently, the antibody may detect both free ubiquitin and ubiquitin conjugates, in addition to the full-length UBA52 protein. Validate specificity in your sample system using appropriate controls.
• For Western blotting, the full-length fusion protein migrates at approximately 15 kDa. Higher molecular weight bands may represent polyubiquitinated species or cross-reactive ubiquitin–protein conjugates.
• In immunohistochemistry and immunofluorescence, expect both cytoplasmic and nuclear staining. Optimize fixation and antigen retrieval conditions for your tissue type.
• For ELISA applications, use the antibody to capture or detect UBA52 or ubiquitinated proteins, ensuring proper blocking to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting researchers and diagnostic manufacturers with validated antibodies, bulk ancillary reagents, and custom raw-material supply. For ubiquitin and ribosome biology studies, we offer a curated portfolio of monoclonal and polyclonal antibodies, recombinant proteins, and technical support to streamline your assay development from concept to clinic.