Anti-UBA3 Rabbit Monoclonal Antibody for WB, ELISA - Q8TBC4

Core Product Specifications and Parameters

Biological Background: UBA3 Function and Localization

• UBA3, also known as NAE2, UBE1C, or ubiquitin-like modifier-activating enzyme 3, is the catalytic subunit of the dimeric NEDD8-activating E1 enzyme.
• It initiates neddylation by adenylating NEDD8's C-terminal glycine with ATP, forming a NEDD8–UBA3 thioester intermediate, and then transfers NEDD8 to the E2 enzyme UBE2M.
• UBA3 is necessary for cell cycle progression and down-regulates steroid receptor activity.
• Ubiquitously expressed across tissues, indicating a housekeeping role in cellular neddylation.
• The protein contains ATP-binding and nucleotide-binding domains and functions as a ligase in the ubiquitin-like conjugation pathway.
• Keywords include acetylation and alternative splicing, suggesting post-translational modifications and isoform diversity.
• Subcellular localization is not annotated in the provided UniProt record but is expected to be cytoplasmic and/or nuclear based on its role in protein modification.

Experimental Guidance and Technical Tips

• For Western blotting, a primary band at ~52 kDa is expected; use standard SDS-PAGE and transfer protocols with appropriate positive and negative controls.
• Validate the antibody in your specific lysate system (e.g., HeLa, HEK293, mouse or rat cell lines) to confirm reactivity.
• For ELISA, perform antibody titration and test direct or indirect assay formats to optimize signal-to-noise ratio.
• Since the immunogen spans nearly full-length UBA3, the clone likely recognizes total UBA3, but isoform specificity should be experimentally confirmed if needed.
• Always handle and store the antibody according to the supplier’s recommendations (shipped on ice bags) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing platform for IVD raw materials. This UBA3 rabbit monoclonal antibody is part of our extensive portfolio of validated antibodies for ubiquitination and cell cycle research. Beyond single antibodies, we offer matched antibody pairs, bulk ancillary reagents, and assistance sourcing rare or custom raw materials—helping you bridge from concept to clinic with reliable supply chains and technical support.